BMRB Entry 10139
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10139
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Title: Backbone NMR assignments of RimM from Thermus thermophilus PubMed: 17616598
Deposition date: 2007-06-13 Original release date: 2008-06-12
Authors: Suzuki, S.; Tatsuguchi, A.; Matsumoto, E.; Kawazoe, M.; Kaminishi, T.; Shirouzu, M.; Muto, Y.; Takemoto, C.; Yokoyama, S.
Citation: Suzuki, S.; Tatsuguchi, A.; Matsumoto, E.; Kawazoe, M.; Kaminishi, T.; Shirouzu, M.; Muto, Y.; Takemoto, C.; Yokoyama, S.. "Structural characterization of the ribosome maturation protein, RimM" J. Bacteriol. 189, .-6397 (6406).
Assembly members:
RimM, polymer, 162 residues, Formula weight is not available
Natural source: Common Name: Thermus thermophilus Taxonomy ID: 274 Superkingdom: Bacteria Kingdom: not available Genus/species: Thermus thermophilus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
RimM: MRLVEIGRFGAPYALKGGLR
FRGEPVVLHLERVYVEGHGW
RAIEDLYRVGEELVVHLAGV
TDRTLAEALVGLRVYAEVAD
LPPLEEGRYYYFALIGLPVY
VEGRQVGEVVDILDAGAQDV
LIIRGVGERLRDRAERLVPL
QAPYVRVEEGSIHVDPIPGL
FD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 413 |
| 15N chemical shifts | 136 |
| 1H chemical shifts | 136 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RimM | 1 |
Entities:
Entity 1, RimM 162 residues - Formula weight is not available
| 1 | MET | ARG | LEU | VAL | GLU | ILE | GLY | ARG | PHE | GLY | ||||
| 2 | ALA | PRO | TYR | ALA | LEU | LYS | GLY | GLY | LEU | ARG | ||||
| 3 | PHE | ARG | GLY | GLU | PRO | VAL | VAL | LEU | HIS | LEU | ||||
| 4 | GLU | ARG | VAL | TYR | VAL | GLU | GLY | HIS | GLY | TRP | ||||
| 5 | ARG | ALA | ILE | GLU | ASP | LEU | TYR | ARG | VAL | GLY | ||||
| 6 | GLU | GLU | LEU | VAL | VAL | HIS | LEU | ALA | GLY | VAL | ||||
| 7 | THR | ASP | ARG | THR | LEU | ALA | GLU | ALA | LEU | VAL | ||||
| 8 | GLY | LEU | ARG | VAL | TYR | ALA | GLU | VAL | ALA | ASP | ||||
| 9 | LEU | PRO | PRO | LEU | GLU | GLU | GLY | ARG | TYR | TYR | ||||
| 10 | TYR | PHE | ALA | LEU | ILE | GLY | LEU | PRO | VAL | TYR | ||||
| 11 | VAL | GLU | GLY | ARG | GLN | VAL | GLY | GLU | VAL | VAL | ||||
| 12 | ASP | ILE | LEU | ASP | ALA | GLY | ALA | GLN | ASP | VAL | ||||
| 13 | LEU | ILE | ILE | ARG | GLY | VAL | GLY | GLU | ARG | LEU | ||||
| 14 | ARG | ASP | ARG | ALA | GLU | ARG | LEU | VAL | PRO | LEU | ||||
| 15 | GLN | ALA | PRO | TYR | VAL | ARG | VAL | GLU | GLU | GLY | ||||
| 16 | SER | ILE | HIS | VAL | ASP | PRO | ILE | PRO | GLY | LEU | ||||
| 17 | PHE | ASP |
Samples:
sample_1: RimM, [U-13C; U-15N], 0.2 mM; HEPES 20 mM; D2O 10%; H2O 90%; NaCl 300 mM
condition_1: ionic strength: 320 mM; pH: 7.0; pressure: 1 atm; temperature: 318 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCA | sample_1 | not available | condition_1 |
| 3D HN(CO)CA | sample_1 | not available | condition_1 |
| 3D HNCO | sample_1 | not available | condition_1 |
| 3D HN(CA)CO | sample_1 | not available | condition_1 |
| 3D CBCA(CO)NH | sample_1 | not available | condition_1 |
| 3D HNCACB | sample_1 | not available | condition_1 |
| 3D C(CO)NH | sample_1 | not available | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9321, Kobayashi, N. - data analysis
Olivia v1.10.5, Yokochi, M. - data analysis
SPARKY v3.110, Goddard, T.D., Kneller, D.G. - data analysis
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| BMRB | 10138 10140 |
| PDB | |
| DBJ | BAD70856 |
| GB | AAS81019 AEG33454 AFH38925 EIA39096 |
| REF | WP_008632437 WP_011228392 YP_144299 |
| SP | Q5SJH5 Q72JU7 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts