BMRB Entry 11099
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11099
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Title: Solution structure of the fourth fibronectin type III domain of human Netrin receptor DCC
Deposition date: 2010-02-18 Original release date: 2011-02-17
Authors: Tochio, N.; Koshiba, S.; Kigawa, T.; Yokoyama, S.
Citation: Tochio, N.; Koshiba, S.; Kigawa, T.; Yokoyama, S.. "Solution structure of the fourth fibronectin type III domain of human Netrin receptor DCC" . ., .-..
Assembly members:
fourth fn3 domain, polymer, 116 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
fourth fn3 domain: GSSGSSGPENDLDESQVPDQ
PSSLHVRPQTNCIIMSWTPP
LNPNIVVRGYIIGYGVGSPY
AETVRVDSKQRYYSIERLES
SSHYVISLKAFNNAGEGVPL
YESATTRSITSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 467 |
| 15N chemical shifts | 108 |
| 1H chemical shifts | 716 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | fourth fn3 domain | 1 |
Entities:
Entity 1, fourth fn3 domain 116 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | PRO | GLU | ASN | ||||
| 2 | ASP | LEU | ASP | GLU | SER | GLN | VAL | PRO | ASP | GLN | ||||
| 3 | PRO | SER | SER | LEU | HIS | VAL | ARG | PRO | GLN | THR | ||||
| 4 | ASN | CYS | ILE | ILE | MET | SER | TRP | THR | PRO | PRO | ||||
| 5 | LEU | ASN | PRO | ASN | ILE | VAL | VAL | ARG | GLY | TYR | ||||
| 6 | ILE | ILE | GLY | TYR | GLY | VAL | GLY | SER | PRO | TYR | ||||
| 7 | ALA | GLU | THR | VAL | ARG | VAL | ASP | SER | LYS | GLN | ||||
| 8 | ARG | TYR | TYR | SER | ILE | GLU | ARG | LEU | GLU | SER | ||||
| 9 | SER | SER | HIS | TYR | VAL | ILE | SER | LEU | LYS | ALA | ||||
| 10 | PHE | ASN | ASN | ALA | GLY | GLU | GLY | VAL | PRO | LEU | ||||
| 11 | TYR | GLU | SER | ALA | THR | THR | ARG | SER | ILE | THR | ||||
| 12 | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: fourth fn3 domain, [U-13C; U-15N], 1.2 mM; d-Tris-HCl, [U-2H], 20 mM; sodium chloride 100 mM; d-DTT, [U-2H], 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_1 | isotropic | condition_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMNMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.955, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - structure solution
NMR spectrometers:
- Bruker AVANCE 900 MHz
Related Database Links:
| PDB | |
| DBJ | BAG63884 BAI45499 |
| EMBL | CAA53735 |
| GB | AAH36524 AAI52809 EAW62991 EAW62994 EFB26812 |
| REF | NP_005206 XP_001916613 XP_002757292 XP_002800982 XP_002828277 |
| SP | P43146 |
| TPG | DAA15913 |
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