BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11101

Title: The solution structure of the first thioredoxin domain of mouse Protein disulfide-isomerase A4

Deposition date: 2010-02-18 Original release date: 2011-02-17

Authors: Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "The solution structure of the first thioredoxin domain of mouse Protein disulfide-isomerase A4"  . ., .-..

Assembly members:
1st thioredoxin domain, polymer, 140 residues, Formula weight is not available

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free

Entity Sequences (FASTA):
1st thioredoxin domain: GSSGSSGDDDLEVKEENGVW VLNDGNFDNFVADKDTVLLE FYAPWCGHCKQFAPEYEKIA STLKDNDPPIAVAKIDATSA SMLASKFDVSGYPTIKILKK GQAVDYDGSRTQEEIVAKVR EVSQPDWTPPPEVTSGPSSG

Data sets:
Data typeCount
13C chemical shifts600
15N chemical shifts137
1H chemical shifts903

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
11st thioredoxin domain1

Entities:

Entity 1, 1st thioredoxin domain 140 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASPASPASP
2   LEUGLUVALLYSGLUGLUASNGLYVALTRP
3   VALLEUASNASPGLYASNPHEASPASNPHE
4   VALALAASPLYSASPTHRVALLEULEUGLU
5   PHETYRALAPROTRPCYSGLYHISCYSLYS
6   GLNPHEALAPROGLUTYRGLULYSILEALA
7   SERTHRLEULYSASPASNASPPROPROILE
8   ALAVALALALYSILEASPALATHRSERALA
9   SERMETLEUALASERLYSPHEASPVALSER
10   GLYTYRPROTHRILELYSILELEULYSLYS
11   GLYGLNALAVALASPTYRASPGLYSERARG
12   THRGLNGLUGLUILEVALALALYSVALARG
13   GLUVALSERGLNPROASPTRPTHRPROPRO
14   PROGLUVALTHRSERGLYPROSERSERGLY

Samples:

sample_1: Thioredoxin domain, [U-13C; U-15N], 1.2 mM; d-Tris-HCl, [U-2H], 20 mM; sodium chloride 100 mM; d-DTT, [U-2H], 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-SEPARATED NOESYsample_1isotropicsample_conditions_1
3D 13C-SEPARATED NOESYsample_1isotropicsample_conditions_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.955, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 900 MHz

Related Database Links:

PDB

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SPARKY: Backbone or all simulated shifts