BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11200

Title: Solution structure of the PDZ domain of Pals1 protein

Deposition date: 2010-07-22 Original release date: 2011-07-21

Authors: Qin, X.; Hayashi, F.; Yokoyama, S.

Citation: Qin, X.; Hayashi, F.; Yokoyama, S.. "Solution structure of the PDZ domain of Pals1 protein"  . ., .-..

Assembly members:
PDZ domain, polymer, 113 residues, Formula weight is not available

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free

Entity Sequences (FASTA):
PDZ domain: GSSGSSGPITDERVYESIGH YGGETVKIVRIEKARDIPLG ATVRNEMDSVIISRIVKGGA AEKSGLLHEGDEVLEINGIE IRGKDVNEVFDLLSDMHGTL TFVLIPSSGPSSG

Data sets:
Data typeCount
13C chemical shifts478
15N chemical shifts114
1H chemical shifts766

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MAGUK p55 subfamily member 51

Entities:

Entity 1, MAGUK p55 subfamily member 5 113 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYPROILETHR
2   ASPGLUARGVALTYRGLUSERILEGLYHIS
3   TYRGLYGLYGLUTHRVALLYSILEVALARG
4   ILEGLULYSALAARGASPILEPROLEUGLY
5   ALATHRVALARGASNGLUMETASPSERVAL
6   ILEILESERARGILEVALLYSGLYGLYALA
7   ALAGLULYSSERGLYLEULEUHISGLUGLY
8   ASPGLUVALLEUGLUILEASNGLYILEGLU
9   ILEARGGLYLYSASPVALASNGLUVALPHE
10   ASPLEULEUSERASPMETHISGLYTHRLEU
11   THRPHEVALLEUILEPROSERSERGLYPRO
12   SERSERGLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.05 mM; PiNa 20 mM; NaCl 100 mM; d10-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20020425, Delaglio.F. - processing

NMRView v5.0.4, JohnsonB.A. - data analysis

Kujira v0.854, KobayashiN. - data analysis

CYANA v1.0.7, GuntertP. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAB14172 BAC05295 BAF83929 BAG11109
EMBL CAD38620 CAD89937 CAH90105 CAL37477 CAL37737
GB AAF63789 AAH53366 AAH95485 AAI29934 AAI38625
REF NP_001101504 NP_001125010 NP_001192880 NP_001243479 NP_062525
SP Q5RDQ2 Q8N3R9 Q9JLB2
TPG DAA25125

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts