BMRB Entry 11254
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11254
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Title: Solution Structure of a N-terminal Ubiquitin-like Domain in Mouse Tubulin-specific Chaperone B
Deposition date: 2010-08-09 Original release date: 2011-08-17
Authors: Zhao, C.; Kigawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation: Zhao, C.; Kigawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution Structure of a N-terminal Ubiquitin-like Domain in Mouse Tubulin-specific Chaperone B" . ., .-..
Assembly members:
Ubiquitin-like domain, polymer, 95 residues, Formula weight is not available
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
Ubiquitin-like domain: GSSGSSGVMVFISSSLNSFR
SEKRYSRSLTIAEFKCKLEL
VVGSPASCMELELYGADDKF
YSKLDQEDALLGSYPVDDGC
RIHVIDHSGSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 402 |
| 15N chemical shifts | 96 |
| 1H chemical shifts | 614 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ubiquitin-like domain | 1 |
Entities:
Entity 1, Ubiquitin-like domain 95 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | VAL | MET | VAL | ||||
| 2 | PHE | ILE | SER | SER | SER | LEU | ASN | SER | PHE | ARG | ||||
| 3 | SER | GLU | LYS | ARG | TYR | SER | ARG | SER | LEU | THR | ||||
| 4 | ILE | ALA | GLU | PHE | LYS | CYS | LYS | LEU | GLU | LEU | ||||
| 5 | VAL | VAL | GLY | SER | PRO | ALA | SER | CYS | MET | GLU | ||||
| 6 | LEU | GLU | LEU | TYR | GLY | ALA | ASP | ASP | LYS | PHE | ||||
| 7 | TYR | SER | LYS | LEU | ASP | GLN | GLU | ASP | ALA | LEU | ||||
| 8 | LEU | GLY | SER | TYR | PRO | VAL | ASP | ASP | GLY | CYS | ||||
| 9 | ARG | ILE | HIS | VAL | ILE | ASP | HIS | SER | GLY | SER | ||||
| 10 | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: Ubiquitin-like domain, [U-13C; U-15N], 1.15 mM; Phosphate Na 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298.0 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B., A. - data analysis
Kujira v0.860, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB22009 BAB22918 BAB26939 |
| GB | AAH10684 EDL24039 EDL24040 ELK17774 |
| REF | NP_079824 XP_005361319 XP_006906006 XP_007096876 XP_011380142 |
| SP | Q9D1E6 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
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