BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11261

Title: Solution Structure of the N-terminal Ubiquitin-like Domain in the Human Ubiquilin 3 (UBQLN3)

Deposition date: 2010-08-09 Original release date: 2011-08-18

Authors: Zhao, C.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Zhao, C.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the N-terminal Ubiquitin-like Domain in the Human Ubiquilin 3 (UBQLN3)"  . ., .-..

Assembly members:
ubiquitin-like fold, polymer, 106 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free

Entity Sequences (FASTA):
ubiquitin-like fold: GSSGSSGSPAPVQDPHLIKV TVKTPKDKEDFSVTDTCTIQ QLKEEISQRFKAHPDQLVLI FAGKILKDPDSLAQCGVRDG LTVHLVIKRQHRAMGNECPA SGPSSG

Data sets:
Data typeCount
13C chemical shifts434
15N chemical shifts97
1H chemical shifts681

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ubiquitin-like fold1

Entities:

Entity 1, ubiquitin-like fold 106 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYSERPROALA
2   PROVALGLNASPPROHISLEUILELYSVAL
3   THRVALLYSTHRPROLYSASPLYSGLUASP
4   PHESERVALTHRASPTHRCYSTHRILEGLN
5   GLNLEULYSGLUGLUILESERGLNARGPHE
6   LYSALAHISPROASPGLNLEUVALLEUILE
7   PHEALAGLYLYSILELEULYSASPPROASP
8   SERLEUALAGLNCYSGLYVALARGASPGLY
9   LEUTHRVALHISLEUVALILELYSARGGLN
10   HISARGALAMETGLYASNGLUCYSPROALA
11   SERGLYPROSERSERGLY

Samples:

sample_1: ubiquitin-like fold, [U-13C; U-15N], 2.0 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298.0 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.921, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

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