BMRB Entry 11441
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11441
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Title: NMR chemical shift of bryum coronatum chitinase PubMed: 21367878
Deposition date: 2011-05-02 Original release date: 2011-06-09
Authors: Shinya, Shoko; Ohnuma, Takayuki; Taira, Toki; Nishimura, Shigenori; Fukamizo, Tamo
Citation: Taira, Toki; Mahoe, Yoko; Kawamoto, Noriko; Onaga, Shoko; Iwasaki, Hironori; Ohnuma, Takayuki; Fukamizo, Tamo. "Cloning and characterization of a small family 19 chitinase from moss (Bryum coronatum)." Glycobiology 21, 644-654 (2011).
Assembly members:
Bryum coronatum chitinase, polymer, 205 residues, Formula weight is not available
Natural source: Common Name: Moss Taxonomy ID: 216087 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Bryum coronatum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Bryum coronatum chitinase: QSWTSFVTPAVFEGWFPNRN
PFYTYDGLVSASNGYPEFGT
TGSLDDQKRELAAFLGNINQ
ESGGLQFIQEQNPQSDYCDT
SSTQYPCAAGKQYYGRGPIQ
LSWNYNYGEAGADLGLDLLN
NPDLVAQDSTVAWRTALWFW
MKRDCHGAITASPPSFSGTI
RIINGGLECNQPAGSIGNMQ
MENRVTYYTQFCQTLGVDPG
TDLRC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 578 |
| 15N chemical shifts | 192 |
| 1H chemical shifts | 192 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Bryum coronatum chitinase | 1 |
Entities:
Entity 1, Bryum coronatum chitinase 205 residues - Formula weight is not available
| 1 | GLN | SER | TRP | THR | SER | PHE | VAL | THR | PRO | ALA | ||||
| 2 | VAL | PHE | GLU | GLY | TRP | PHE | PRO | ASN | ARG | ASN | ||||
| 3 | PRO | PHE | TYR | THR | TYR | ASP | GLY | LEU | VAL | SER | ||||
| 4 | ALA | SER | ASN | GLY | TYR | PRO | GLU | PHE | GLY | THR | ||||
| 5 | THR | GLY | SER | LEU | ASP | ASP | GLN | LYS | ARG | GLU | ||||
| 6 | LEU | ALA | ALA | PHE | LEU | GLY | ASN | ILE | ASN | GLN | ||||
| 7 | GLU | SER | GLY | GLY | LEU | GLN | PHE | ILE | GLN | GLU | ||||
| 8 | GLN | ASN | PRO | GLN | SER | ASP | TYR | CYS | ASP | THR | ||||
| 9 | SER | SER | THR | GLN | TYR | PRO | CYS | ALA | ALA | GLY | ||||
| 10 | LYS | GLN | TYR | TYR | GLY | ARG | GLY | PRO | ILE | GLN | ||||
| 11 | LEU | SER | TRP | ASN | TYR | ASN | TYR | GLY | GLU | ALA | ||||
| 12 | GLY | ALA | ASP | LEU | GLY | LEU | ASP | LEU | LEU | ASN | ||||
| 13 | ASN | PRO | ASP | LEU | VAL | ALA | GLN | ASP | SER | THR | ||||
| 14 | VAL | ALA | TRP | ARG | THR | ALA | LEU | TRP | PHE | TRP | ||||
| 15 | MET | LYS | ARG | ASP | CYS | HIS | GLY | ALA | ILE | THR | ||||
| 16 | ALA | SER | PRO | PRO | SER | PHE | SER | GLY | THR | ILE | ||||
| 17 | ARG | ILE | ILE | ASN | GLY | GLY | LEU | GLU | CYS | ASN | ||||
| 18 | GLN | PRO | ALA | GLY | SER | ILE | GLY | ASN | MET | GLN | ||||
| 19 | MET | GLU | ASN | ARG | VAL | THR | TYR | TYR | THR | GLN | ||||
| 20 | PHE | CYS | GLN | THR | LEU | GLY | VAL | ASP | PRO | GLY | ||||
| 21 | THR | ASP | LEU | ARG | CYS |
Samples:
sample_1: BcChiA, [U-99% 13C; U-99% 15N], 0.4 mM; sodium acetate 50 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 5.0; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCOCA | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 500 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts