BMRB Entry 11516
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11516
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Title: THE SOLUTION STRUCTURE OF THE C-TERMINAL DOMAIN OF HUMAN ACTIVATOR OF 90 KDA HEAT SHOCK PROTEIN ATPASE HOMOLOG 1
Deposition date: 2012-11-19 Original release date: 2012-12-10
Authors: Tochio, Naoya; Koshiba, Seizo; Inoue, Makoto; Kigawa, Takanori; Yokoyama, Shigeyuki
Citation: Tochio, Naoya; Koshiba, Seizo; Kigawa, Takanori; Yokoyama, Shigeyuki. "THE SOLUTION STRUCTURE OF THE C-TERMINAL DOMAIN OF HUMAN ACTIVATOR OF 90 KDA HEAT SHOCK PROTEIN ATPASE HOMOLOG 1" Not known ., .-..
Assembly members:
protein, polymer, 145 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: E. coli - cell free
Entity Sequences (FASTA):
protein: GSSGSSGIPTCKITLKETFL
TSPEELYRVFTTQELVQAFT
HAPATLEADRGGKFHMVDGN
VSGEFTDLVPEKHIVMKWRF
KSWPEGHFATITLTFIDKNG
ETELCMEGRGIPAPEEERTR
QGWQRYYFEGIKQTFGYGAS
GPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 639 |
| 15N chemical shifts | 138 |
| 1H chemical shifts | 957 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AHA1 C-terminal domain | 1 |
Entities:
Entity 1, AHA1 C-terminal domain 145 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ILE | PRO | THR | ||||
| 2 | CYS | LYS | ILE | THR | LEU | LYS | GLU | THR | PHE | LEU | ||||
| 3 | THR | SER | PRO | GLU | GLU | LEU | TYR | ARG | VAL | PHE | ||||
| 4 | THR | THR | GLN | GLU | LEU | VAL | GLN | ALA | PHE | THR | ||||
| 5 | HIS | ALA | PRO | ALA | THR | LEU | GLU | ALA | ASP | ARG | ||||
| 6 | GLY | GLY | LYS | PHE | HIS | MET | VAL | ASP | GLY | ASN | ||||
| 7 | VAL | SER | GLY | GLU | PHE | THR | ASP | LEU | VAL | PRO | ||||
| 8 | GLU | LYS | HIS | ILE | VAL | MET | LYS | TRP | ARG | PHE | ||||
| 9 | LYS | SER | TRP | PRO | GLU | GLY | HIS | PHE | ALA | THR | ||||
| 10 | ILE | THR | LEU | THR | PHE | ILE | ASP | LYS | ASN | GLY | ||||
| 11 | GLU | THR | GLU | LEU | CYS | MET | GLU | GLY | ARG | GLY | ||||
| 12 | ILE | PRO | ALA | PRO | GLU | GLU | GLU | ARG | THR | ARG | ||||
| 13 | GLN | GLY | TRP | GLN | ARG | TYR | TYR | PHE | GLU | GLY | ||||
| 14 | ILE | LYS | GLN | THR | PHE | GLY | TYR | GLY | ALA | SER | ||||
| 15 | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: protein, [U-13C; U-15N], 1.3 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
xwinnmr, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - data analysis
Kujira, N. Kobayashi - data analysis
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Bruker Avance 900 MHz
Related Database Links:
| PDB | |
| DBJ | BAC36160 BAE38913 BAG36559 BAG62431 |
| EMBL | CAB45684 CAH92050 |
| GB | AAD09623 AAF29000 AAF80755 AAH00321 AAH07398 |
| REF | NP_001108506 NP_001127499 NP_001253813 NP_036243 NP_666148 |
| SP | O95433 Q8BK64 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts