BMRB Entry 15091

Name: Lipid Free Mouse Apolipoprotein-AI(mAI216)NMR Backbone Assignment
Published: 2007-08-22

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR15091

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Lipid Free Mouse Apolipoprotein-AI(mAI216)NMR Backbone Assignment PubMed: 19636841

Deposition date: 2007-01-02 Original release date: 2007-08-22

Authors: Yang, Yunhuang; Wang, Jianjun

Citation: Yang, Yunhuang; Hoyt, David; Wang, Jianjun. "A complete NMR spectral assignment of the lipid-free mouse apolipoprotein A-I (apoAI) C-terminal truncation mutant, apoAI(1-216)" Biomol. NMR Assignments 1, 109-111 (2007).

Assembly members:
apoprotein_AI, polymer, 240 residues, Formula weight is not available

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
apoprotein_AI: DEPQSQWDKVKDFANVYVDA VKDSGRDYVSQFESSSLGQQ LNLNLLENWDTLGSTVSQLQ ERLGPLTRDFWDNLEKETDW VRQEMNKDLEEVKQKVQPYL DEFQKKWKEDVELYRQKASP QGAELQESARQKLQELQGRL SPVAEEFRDRMRTHVDSLRT QLAPHSEQMRESLAQRLAEL KSNPTLNEYHSSAKSHLKSL GEKARPALEDLRHSLMPQLE SLKTKAQSEIDKASENLNAQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	641
15N chemical shifts	194
1H chemical shifts	1045

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	mApoAI216	1

Entities:

Entity 1, mApoAI216 240 residues - Formula weight is not available

1	ASP	GLU	PRO	GLN	SER	GLN	TRP	ASP	LYS	VAL
2	LYS	ASP	PHE	ALA	ASN	VAL	TYR	VAL	ASP	ALA
3	VAL	LYS	ASP	SER	GLY	ARG	ASP	TYR	VAL	SER
4	GLN	PHE	GLU	SER	SER	SER	LEU	GLY	GLN	GLN
5	LEU	ASN	LEU	ASN	LEU	LEU	GLU	ASN	TRP	ASP
6	THR	LEU	GLY	SER	THR	VAL	SER	GLN	LEU	GLN
7	GLU	ARG	LEU	GLY	PRO	LEU	THR	ARG	ASP	PHE
8	TRP	ASP	ASN	LEU	GLU	LYS	GLU	THR	ASP	TRP
9	VAL	ARG	GLN	GLU	MET	ASN	LYS	ASP	LEU	GLU
10	GLU	VAL	LYS	GLN	LYS	VAL	GLN	PRO	TYR	LEU
11	ASP	GLU	PHE	GLN	LYS	LYS	TRP	LYS	GLU	ASP
12	VAL	GLU	LEU	TYR	ARG	GLN	LYS	ALA	SER	PRO
13	GLN	GLY	ALA	GLU	LEU	GLN	GLU	SER	ALA	ARG
14	GLN	LYS	LEU	GLN	GLU	LEU	GLN	GLY	ARG	LEU
15	SER	PRO	VAL	ALA	GLU	GLU	PHE	ARG	ASP	ARG
16	MET	ARG	THR	HIS	VAL	ASP	SER	LEU	ARG	THR
17	GLN	LEU	ALA	PRO	HIS	SER	GLU	GLN	MET	ARG
18	GLU	SER	LEU	ALA	GLN	ARG	LEU	ALA	GLU	LEU
19	LYS	SER	ASN	PRO	THR	LEU	ASN	GLU	TYR	HIS
20	SER	SER	ALA	LYS	SER	HIS	LEU	LYS	SER	LEU
21	GLY	GLU	LYS	ALA	ARG	PRO	ALA	LEU	GLU	ASP
22	LEU	ARG	HIS	SER	LEU	MET	PRO	GLN	LEU	GLU
23	SER	LEU	LYS	THR	LYS	ALA	GLN	SER	GLU	ILE
24	ASP	LYS	ALA	SER	GLU	ASN	LEU	ASN	ALA	GLN

Samples:

triple_labeled_mAI216: apoprotein AI, [U-13C; U-15N; U-2H], 1.5 mM; D2O, [U-99% 2H], 5%; DSS 0.1 mM; H2O 95%; sodium phosphate 25 mM; EDTA 10 mM; sodium chloride 25 mM; sodium azide 1 mM

sample_conditions_1: ionic strength: 0.08 M; pH: 6.8; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	triple_labeled_mAI216	isotropic	sample_conditions_1
3D HNCA	triple_labeled_mAI216	isotropic	sample_conditions_1
3D HN(CO)CA	triple_labeled_mAI216	isotropic	sample_conditions_1
3D HN(CA)CB	triple_labeled_mAI216	isotropic	sample_conditions_1
3D HN(COCA)CB	triple_labeled_mAI216	isotropic	sample_conditions_1
3D 1H-15N NOESY	triple_labeled_mAI216	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Zhengrong and Bax - processing

NMR spectrometers:

Varian INOVA 600 MHz
Varian Unity 900 MHz

BMRB	17717
PDB	2LEM

Biological Magnetic Resonance Data Bank