BMRB Entry 15200
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15200
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Title: NMR assignment of the apo-form of a Desulfovibrio gigas protein containing a novel Mo-Cu cluster PubMed: 19636833
Deposition date: 2007-04-03 Original release date: 2007-08-23
Authors: Pauleta, Sofia; Moura, Jose
Citation: Pauleta, Sofia; Duarte, Americo; Carepo, Marta; Pereira, Alice; Tavares, Pedro; Moura, Isabel; Moura, Jose. "NMR assignment of the apo-form of a Desulfovibrio gigas protein containing a novel Mo-Cu cluster" Biomol. NMR Assignments 1, 81-83 (2007).
Assembly members:
ApoORP, polymer, 120 residues, Formula weight is not available
Natural source: Common Name: Desulfovibrio gigas Taxonomy ID: 879 Superkingdom: Bacteria Kingdom: not available Genus/species: Desulfovibrio gigas
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ApoORP: ASHMQRIAVTAEGPGLDGLV
DPRFGRAAGFVVVDAATMAA
EYVDNGASQTLSHGAGINAA
QVLAKSGAGVLLTGYVGPKA
FQALQAAGIKVGQDLEGLTV
RQAVQRFLDGQVPMAAGPNK
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 523 |
15N chemical shifts | 135 |
1H chemical shifts | 773 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | subunit 1 | 1 |
Entities:
Entity 1, subunit 1 120 residues - Formula weight is not available
1 | ALA | SER | HIS | MET | GLN | ARG | ILE | ALA | VAL | THR | |
2 | ALA | GLU | GLY | PRO | GLY | LEU | ASP | GLY | LEU | VAL | |
3 | ASP | PRO | ARG | PHE | GLY | ARG | ALA | ALA | GLY | PHE | |
4 | VAL | VAL | VAL | ASP | ALA | ALA | THR | MET | ALA | ALA | |
5 | GLU | TYR | VAL | ASP | ASN | GLY | ALA | SER | GLN | THR | |
6 | LEU | SER | HIS | GLY | ALA | GLY | ILE | ASN | ALA | ALA | |
7 | GLN | VAL | LEU | ALA | LYS | SER | GLY | ALA | GLY | VAL | |
8 | LEU | LEU | THR | GLY | TYR | VAL | GLY | PRO | LYS | ALA | |
9 | PHE | GLN | ALA | LEU | GLN | ALA | ALA | GLY | ILE | LYS | |
10 | VAL | GLY | GLN | ASP | LEU | GLU | GLY | LEU | THR | VAL | |
11 | ARG | GLN | ALA | VAL | GLN | ARG | PHE | LEU | ASP | GLY | |
12 | GLN | VAL | PRO | MET | ALA | ALA | GLY | PRO | ASN | LYS |
Samples:
sample_1: ApoORP, [U-15N], 1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 1 mM; D2O 10%
sample_2: ApoORP, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 1 mM; D2O 10%
sample_conditions_1: ionic strength: 120 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_2 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_2 | isotropic | sample_conditions_1 |
3D HNHA | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_2 | isotropic | sample_conditions_1 |
3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
CARA, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 600 MHz
- Bruker Avance 700 MHz
- Bruker Avance 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts