BMRB Entry 15247
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15247
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Title: 1H, 13C and 15N resonance assignments of the Escherichia coli YaeT POTRA domain PubMed: 19636842
Deposition date: 2007-05-14 Original release date: 2007-08-22
Authors: Knowles, Timothy; Bobat, Saeeda; Jeeves, Mark; Henderson, Ian; Overduin, Michael
Citation: Knowles, Timothy; Bobat, Saeeda; Jeeves, Mark; Henderson, Ian; Overduin, Michael. "Secondary structure and 1H, 13C and 15N resonance assignments of the Escherichia coli YaeT POTRA domain" Biomol. NMR Assignments 1, 113-115 (2007).
Assembly members:
POTRA, polymer, 164 residues, 17967.2 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
POTRA: AEGFVVKDIHFEGLQRVAVG
AALLSMPVRTGDTVNDEDIS
NTIRALFATGNFEDVRVLRD
GDTLLVQVKERPTIASITFS
GNKSVKDDMLKQNLEASGVR
VGESLDRTTIADIEKGLEDF
YYSVGKYSASVKAVVTPLPR
NRVDLKLVFQEGVSLQHHHH
QTAG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 669 |
| 15N chemical shifts | 159 |
| 1H chemical shifts | 1078 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | POTRA | 1 |
Entities:
Entity 1, POTRA 164 residues - 17967.2 Da.
Residues 1-156 represent the first two POTRA domains of YaeT. Residues 157-164 non-native affinity tag
| 1 | ALA | GLU | GLY | PHE | VAL | VAL | LYS | ASP | ILE | HIS | ||||
| 2 | PHE | GLU | GLY | LEU | GLN | ARG | VAL | ALA | VAL | GLY | ||||
| 3 | ALA | ALA | LEU | LEU | SER | MET | PRO | VAL | ARG | THR | ||||
| 4 | GLY | ASP | THR | VAL | ASN | ASP | GLU | ASP | ILE | SER | ||||
| 5 | ASN | THR | ILE | ARG | ALA | LEU | PHE | ALA | THR | GLY | ||||
| 6 | ASN | PHE | GLU | ASP | VAL | ARG | VAL | LEU | ARG | ASP | ||||
| 7 | GLY | ASP | THR | LEU | LEU | VAL | GLN | VAL | LYS | GLU | ||||
| 8 | ARG | PRO | THR | ILE | ALA | SER | ILE | THR | PHE | SER | ||||
| 9 | GLY | ASN | LYS | SER | VAL | LYS | ASP | ASP | MET | LEU | ||||
| 10 | LYS | GLN | ASN | LEU | GLU | ALA | SER | GLY | VAL | ARG | ||||
| 11 | VAL | GLY | GLU | SER | LEU | ASP | ARG | THR | THR | ILE | ||||
| 12 | ALA | ASP | ILE | GLU | LYS | GLY | LEU | GLU | ASP | PHE | ||||
| 13 | TYR | TYR | SER | VAL | GLY | LYS | TYR | SER | ALA | SER | ||||
| 14 | VAL | LYS | ALA | VAL | VAL | THR | PRO | LEU | PRO | ARG | ||||
| 15 | ASN | ARG | VAL | ASP | LEU | LYS | LEU | VAL | PHE | GLN | ||||
| 16 | GLU | GLY | VAL | SER | LEU | GLN | HIS | HIS | HIS | HIS | ||||
| 17 | GLN | THR | ALA | GLY |
Samples:
sample_1: POTRA, [U-99% 13C; U-99% 15N], 0.8 mM; MES 50 mM; sodium chloride 50 mM; sodium azide 3 mM; Complete protease inhibitor cocktail 1 tablet/100ml
sample_conditions_1: ionic strength: 86 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(C)CH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D (H)CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY v3.110, Goddard - chemical shift assignment
TALOS v2003.027.13.05, Cornilescu, Delaglio and Bax - data analysis
NMRPipe v2004.194.17.2, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 800 MHz
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA77852 BAB33602 BAG75700 BAI23538 BAI29053 |
| EMBL | CAP74746 CAQ30691 CAQ87780 CAQ97064 CAR01552 |
| GB | AAB08606 AAC73288 AAD23568 AAG54479 AAK64508 |
| REF | NP_308206 NP_414719 NP_706122 WP_001240878 WP_001240879 |
| SP | A1A7M1 A7ZHR7 A7ZWC3 A8ALB1 A9MPI4 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts