BMRB Entry 15498
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15498
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Title: Backbone and side-chain 1H, 13C and 15N resonance assignments for the E2 conjugating enzyme, UbcH7 PubMed: 19636915
Deposition date: 2007-09-27 Original release date: 2008-01-17
Authors: Serniwka, Stephanie; Shaw, Gary
Citation: Serniwka, Stephanie; Shaw, Gary. "1H, 13C and 15N resonance assignments for the human E2 conjugating enzyme, UbcH7" Biomol. NMR Assignments 2, 21-23 (2008).
Assembly members:
UbcH7, polymer, 153 residues, 18684 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
UbcH7: AASRRLMKELEEIRKSGMKN
FRNIQVDEANLLTWQGLIVP
DNPPYDKGAFRIEINFPAEY
PFKPPKITFKTKIYHPNIDE
KGQVCLPVISAENWKPATKT
DQVIQSLIALVNDPQPEHPL
RADLAEEYSKDRKKFSKNAE
EFTKKYGEKRPVD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 602 |
| 15N chemical shifts | 150 |
| 1H chemical shifts | 736 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | UbcH7 | 1 |
Entities:
Entity 1, UbcH7 153 residues - 18684 Da.
| 1 | ALA | ALA | SER | ARG | ARG | LEU | MET | LYS | GLU | LEU | ||||
| 2 | GLU | GLU | ILE | ARG | LYS | SER | GLY | MET | LYS | ASN | ||||
| 3 | PHE | ARG | ASN | ILE | GLN | VAL | ASP | GLU | ALA | ASN | ||||
| 4 | LEU | LEU | THR | TRP | GLN | GLY | LEU | ILE | VAL | PRO | ||||
| 5 | ASP | ASN | PRO | PRO | TYR | ASP | LYS | GLY | ALA | PHE | ||||
| 6 | ARG | ILE | GLU | ILE | ASN | PHE | PRO | ALA | GLU | TYR | ||||
| 7 | PRO | PHE | LYS | PRO | PRO | LYS | ILE | THR | PHE | LYS | ||||
| 8 | THR | LYS | ILE | TYR | HIS | PRO | ASN | ILE | ASP | GLU | ||||
| 9 | LYS | GLY | GLN | VAL | CYS | LEU | PRO | VAL | ILE | SER | ||||
| 10 | ALA | GLU | ASN | TRP | LYS | PRO | ALA | THR | LYS | THR | ||||
| 11 | ASP | GLN | VAL | ILE | GLN | SER | LEU | ILE | ALA | LEU | ||||
| 12 | VAL | ASN | ASP | PRO | GLN | PRO | GLU | HIS | PRO | LEU | ||||
| 13 | ARG | ALA | ASP | LEU | ALA | GLU | GLU | TYR | SER | LYS | ||||
| 14 | ASP | ARG | LYS | LYS | PHE | SER | LYS | ASN | ALA | GLU | ||||
| 15 | GLU | PHE | THR | LYS | LYS | TYR | GLY | GLU | LYS | ARG | ||||
| 16 | PRO | VAL | ASP |
Samples:
sample: UbcH7, [U-100% 13C; U-100% 15N], 0.5 mM; TRIS 25 mM; EDTA 1 mM; DTT 5 mM; sodium chloride 200 mM
Sample_Conditions_for_UbcH7: pH: 7.0; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample | isotropic | Sample_Conditions_for_UbcH7 |
| 2D 1H-13C HSQC | sample | isotropic | Sample_Conditions_for_UbcH7 |
| 3D HCCH-TOCSY | sample | isotropic | Sample_Conditions_for_UbcH7 |
| 3D CBCA(CO)NH | sample | isotropic | Sample_Conditions_for_UbcH7 |
| 3D C(CO)NH | sample | isotropic | Sample_Conditions_for_UbcH7 |
| 3D HNCO | sample | isotropic | Sample_Conditions_for_UbcH7 |
| 3D HNCACB | sample | isotropic | Sample_Conditions_for_UbcH7 |
| 3D H(CCO)NH | sample | isotropic | Sample_Conditions_for_UbcH7 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAB24925 BAE38300 BAE39867 BAE87197 BAF83772 |
| EMBL | CAA04156 CAA10265 CAA63538 CAA65755 CAG30492 |
| GB | AAB36017 AAG17922 AAH53368 AAH77940 AAH93503 |
| REF | NP_001006180 NP_001071562 NP_001086444 NP_001127614 NP_001172062 |
| SP | P68036 P68037 Q3MHP1 Q5R5I4 |
| TPG | DAA20415 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts