BMRB Entry 15577
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15577
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: C Repressor PubMed: 19636908
Deposition date: 2007-12-03 Original release date: 2008-10-14
Authors: Massad, Tariq; Papadopoulos, Evangelos; Damberg, Peter
Citation: Massad, Tariq; Papadopoulos, Evangelos; Henriksson-Peltola, Petri; Haggard-Ljungquist, Elisabeth; Damberg, Peter. "Assignment of 1H, 13C, and 15N chemical shift resonances of P2 C-repressor protein" Biomol. NMR Assignments 2, 215-217 (2008).
Assembly members:
Crepressor, polymer, 99 residues, Formula weight is not available
Natural source: Common Name: Enterobacteria phage P2 Taxonomy ID: 10679 Superkingdom: Viruses Kingdom: not available Genus/species: P2-like viruses Enterobacteria phage P2
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Crepressor: MSNTISEKIVLMRKSEYLSR
QQLADLTGVPYGTLSYYESG
RSTPPTDVMMNILQTPQFTK
YTLWFMTNQIAPESGQIAPA
LAHFGQNETTSPHSGQKTG
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 422 |
15N chemical shifts | 106 |
1H chemical shifts | 670 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Crepressor | 1 |
Entities:
Entity 1, Crepressor 99 residues - Formula weight is not available
1 | MET | SER | ASN | THR | ILE | SER | GLU | LYS | ILE | VAL | ||||
2 | LEU | MET | ARG | LYS | SER | GLU | TYR | LEU | SER | ARG | ||||
3 | GLN | GLN | LEU | ALA | ASP | LEU | THR | GLY | VAL | PRO | ||||
4 | TYR | GLY | THR | LEU | SER | TYR | TYR | GLU | SER | GLY | ||||
5 | ARG | SER | THR | PRO | PRO | THR | ASP | VAL | MET | MET | ||||
6 | ASN | ILE | LEU | GLN | THR | PRO | GLN | PHE | THR | LYS | ||||
7 | TYR | THR | LEU | TRP | PHE | MET | THR | ASN | GLN | ILE | ||||
8 | ALA | PRO | GLU | SER | GLY | GLN | ILE | ALA | PRO | ALA | ||||
9 | LEU | ALA | HIS | PHE | GLY | GLN | ASN | GLU | THR | THR | ||||
10 | SER | PRO | HIS | SER | GLY | GLN | LYS | THR | GLY |
Samples:
sample_1: Crepressor, [U-100% 13C; U-100% 15N], 0.6 mM; sodium phosphate 5 mM; D2O, [U-100% 2H], 10%; H2O 90%
sample_conditions_1: ionic strength: 0.005 M; pH: 6; pressure: 0.7 atm; temperature: 310 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D HNHA | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 900 MHz
- Varian INOVA 800 MHz
- Varian INOVA 600 MHz
- Bruker Avance 500 MHz
Related Database Links:
PDB | |
EMBL | CAJ43636 CAJ43638 CAJ43640 CAJ43642 CAJ43644 |
GB | AAD03298 AAO64734 ABJ01492 AHM44333 AHM48945 |
REF | NP_046787 WP_001306384 WP_001350698 |
SP | P04132 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts