BMRB Entry 15764
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15764
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Ca2+-Binding Domain 1 of the Na+/Ca2+ Exchanger in the Absence of Ca2+ PubMed: 18280495
Deposition date: 2008-05-09 Original release date: 2008-06-30
Authors: Johnson, Eric; Bruschweiler-Li, Lei; Showalter, Scott; Vuister, Geerten; Zhang, Fengli; Bruschweiler, Rafael
Citation: Johnson, Eric; Bruschweiler-Li, Lei; Showalter, Scott; Vuister, Geerten; Zhang, Fengli; Bruschweiler, Rafael. "Structure and Dynamics of Ca2+-Binding Domain 1 of the Na+/Ca2+ Exchanger in the Presence and in the Absence of Ca2+" J. Mol. Biol. 377, 945-955 (2008).
Assembly members:
CBD1, polymer, 147 residues, 16193 Da.
Natural source: Common Name: Dog Taxonomy ID: 9615 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Canis familiaris
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
CBD1: MSHHHHHHVSKIFFEQGTYQ
CLENCGTVALTIIRRGGDLT
NTVFVDFRTEDGTANAGSDY
EFTEGTVVFKPGETQKEIRV
GIIDDDIFEEDENFLVHLSN
VKVSSEASEDGILEANHVSA
LACLGSPSTATVTIFDDDHA
GIFTFEE
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 356 |
15N chemical shifts | 127 |
1H chemical shifts | 127 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | CBD1 | 1 |
Entities:
Entity 1, CBD1 147 residues - 16193 Da.
Residues 1-8 represent a non-native affinity tag.
1 | MET | SER | HIS | HIS | HIS | HIS | HIS | HIS | VAL | SER | ||||
2 | LYS | ILE | PHE | PHE | GLU | GLN | GLY | THR | TYR | GLN | ||||
3 | CYS | LEU | GLU | ASN | CYS | GLY | THR | VAL | ALA | LEU | ||||
4 | THR | ILE | ILE | ARG | ARG | GLY | GLY | ASP | LEU | THR | ||||
5 | ASN | THR | VAL | PHE | VAL | ASP | PHE | ARG | THR | GLU | ||||
6 | ASP | GLY | THR | ALA | ASN | ALA | GLY | SER | ASP | TYR | ||||
7 | GLU | PHE | THR | GLU | GLY | THR | VAL | VAL | PHE | LYS | ||||
8 | PRO | GLY | GLU | THR | GLN | LYS | GLU | ILE | ARG | VAL | ||||
9 | GLY | ILE | ILE | ASP | ASP | ASP | ILE | PHE | GLU | GLU | ||||
10 | ASP | GLU | ASN | PHE | LEU | VAL | HIS | LEU | SER | ASN | ||||
11 | VAL | LYS | VAL | SER | SER | GLU | ALA | SER | GLU | ASP | ||||
12 | GLY | ILE | LEU | GLU | ALA | ASN | HIS | VAL | SER | ALA | ||||
13 | LEU | ALA | CYS | LEU | GLY | SER | PRO | SER | THR | ALA | ||||
14 | THR | VAL | THR | ILE | PHE | ASP | ASP | ASP | HIS | ALA | ||||
15 | GLY | ILE | PHE | THR | PHE | GLU | GLU |
Samples:
sample_1: CBD1, [U-100% 13C; U-100% 15N], 0.5 mM; HEPES 20 mM; beta-mercaptoethanol 20 mM; sodium azide 0.02%; EDTA 15 mM; sodium chloride 100 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 306 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
PDB | |
DBJ | BAF84385 |
EMBL | CAH91585 |
GB | AAA97928 AAB40148 AAD04173 AAD04174 AAD17213 |
REF | NP_001028033 NP_001106272 NP_001106273 NP_001125933 NP_001164429 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts