BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15779

Title: Backbone 1H, 13C and 15N assignments for ginkbilobin-2 with C-terminal His6-tag   PubMed: 19603485

Deposition date: 2008-05-20 Original release date: 2009-10-14

Authors: Miyakawa, Takuya; Sawano, Yoriko; Hatano, Ken-ichi; Tanokura, Masaru

Citation: Miyakawa, Takuya; Miyazono, Ken-ichi; Sawano, Yoriko; Hatano, Ken-ichi; Tanokura, Masaru. "Crystal structure of ginkbilobin-2 with homology to the extracellular domain of plant cysteine-rich receptor-like kinases"  Proteins 77, 247-251 (2009).

Assembly members:
ginkbilobin-2, polymer, 117 residues, Formula weight is not available

Natural source:   Common Name: ginkgo   Taxonomy ID: 3311   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Ginkgo biloba

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ginkbilobin-2: MANTAFVSSACNTQKIPSGS PFNRNLRAMLADLRQNTAFS GYDYKTSRAGSGGAPTAYGR ATCKQSISQSDCTACLSNLV NRIFSICNNAIGARVQLVDC FIQYEQRSFLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts390
15N chemical shifts120
1H chemical shifts135

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ginkbilobin-21

Entities:

Entity 1, ginkbilobin-2 117 residues - Formula weight is not available

1   METALAASNTHRALAPHEVALSERSERALA
2   CYSASNTHRGLNLYSILEPROSERGLYSER
3   PROPHEASNARGASNLEUARGALAMETLEU
4   ALAASPLEUARGGLNASNTHRALAPHESER
5   GLYTYRASPTYRLYSTHRSERARGALAGLY
6   SERGLYGLYALAPROTHRALATYRGLYARG
7   ALATHRCYSLYSGLNSERILESERGLNSER
8   ASPCYSTHRALACYSLEUSERASNLEUVAL
9   ASNARGILEPHESERILECYSASNASNALA
10   ILEGLYALAARGVALGLNLEUVALASPCYS
11   PHEILEGLNTYRGLUGLNARGSERPHELEU
12   GLUHISHISHISHISHISHIS

Samples:

sample_1: ginkbilobin-2, [U-99% 13C; U-99% 15N], 0.95 mM; H2O 92%; D2O 8%

sample_conditions_1: ionic strength: 0 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB ABF55255 ACP27608 ACZ57929
SP A4ZDL6

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts