BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15824

Title: Bank Vole Prion Protein (121-231)   PubMed: 18773909

Deposition date: 2008-06-25 Original release date: 2008-09-12

Authors: Christen, Barbara; Hornemann, Simone; Wuthrich, Kurt

Citation: Christen, Barbara; Perez, Daniel; Hornemann, Simone; Wuthrich, Kurt. "NMR Structure of the Bank Vole Prion Protein at 20 degrees C Contains a Structured Loop of Residues 165-171"  J. Mol. Biol. 383, 306-312 (2008).

Assembly members:
Prion_Protein, polymer, 113 residues, Formula weight is not available

Natural source:   Common Name: Bank vole   Taxonomy ID: 447135   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Clethrionomys glareolus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Prion_Protein: GSVVGGLGGYMLGSAMSRPM IHFGNDWEDRYYRENMNRYP NQVYYRPVDQYNNQNNFVHD CVNITIKQHTVTTTTKGENF TETDVKMMERVVEQMCVTQY QKESQAYYEGRSS

Data sets:
Data typeCount
13C chemical shifts364
15N chemical shifts135
1H chemical shifts414

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Prion Protein1

Entities:

Entity 1, Prion Protein 113 residues - Formula weight is not available

1   GLYSERVALVALGLYGLYLEUGLYGLYTYR
2   METLEUGLYSERALAMETSERARGPROMET
3   ILEHISPHEGLYASNASPTRPGLUASPARG
4   TYRTYRARGGLUASNMETASNARGTYRPRO
5   ASNGLNVALTYRTYRARGPROVALASPGLN
6   TYRASNASNGLNASNASNPHEVALHISASP
7   CYSVALASNILETHRILELYSGLNHISTHR
8   VALTHRTHRTHRTHRLYSGLYGLUASNPHE
9   THRGLUTHRASPVALLYSMETMETGLUARG
10   VALVALGLUGLNMETCYSVALTHRGLNTYR
11   GLNLYSGLUSERGLNALATYRTYRGLUGLY
12   ARGSERSER

Samples:

15N: Prion Protein, [U-99% 15N], 1 mM; D2O 10%; H2O 90%; sodium acetate, [U-2H], 10 mM; H2O 90%; D2O, [U-100% 2H], 10%

13C_15N: Prion Protein, [U-99% 13C; U-99% 15N], 1 mM; D2O 10%; H2O 90%; sodium acetate, [U-2H], 10 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 0.01 M; pH: 4.5; pressure: 1 atm; temperature: 293.2 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESY15Nisotropicsample_conditions_1
3D 1H-13C NOESY13C_15Nisotropicsample_conditions_1

Software:

OPAL v1.2, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

CARA v1.5.5, Keller, R. - data analysis

ATHNOS-CANDID v1.2, Herrmann, Guntert and Wuthrich - chemical shift assignment, peak picking

DYANA v1.0.3, Guntert, Braun and Wuthrich - structure solution

Molmol v2K.2, Koradi, Billeter and Wuthrich - data analysis

xwinnmr, Bruker Biospin - collection

NMR spectrometers:

  • Bruker DRX 750 MHz
  • Bruker Avance 900 MHz

Related Database Links:

BMRB 15845
PDB
GB AAL57231 AAL57232 ABR23642 ACJ06784 ACV85679
REF XP_005365625 XP_006984054 XP_007609107