BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16082

Title: solution structure of FAPP1 PH domain   PubMed: 21454700

Deposition date: 2008-12-22 Original release date: 2010-01-07

Authors: Lenoir, Marc; Coskun, Uenal; James, Jonathan; Simons, Kai; Overduin, Michael

Citation: He, Ju; Scott, Jordan; Heroux, Annie; Roy, Siddhartha; Lenoir, Marc; Overduin, Michael; Stahelin, Robert; Kutateladze, Tatiana. "Molecular basis of phosphatidylinositol 4-phosphate and ARF1 GTPase recognition by the FAPP1 pleckstrin homology (PH) domain."  J. Biol. Chem. 286, 18650-18657 (2011).

Assembly members:
FAPP1-PH, polymer, 108 residues, 12219.981 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
FAPP1-PH: GPLGSPEFMEGVLYKWTNYL TGWQPRWFVLDNGILSYYDS QDDVCKGSKGSIKMAVCEIK VHSADNTRMELIIPGEQHFY MKAVNAAERQRWLVALGSSK ASLTDTRT

Data sets:
Data typeCount
13C chemical shifts465
15N chemical shifts117
1H chemical shifts746

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FAPP1-PH1

Entities:

Entity 1, FAPP1-PH 108 residues - 12219.981 Da.

1   GLYPROLEUGLYSERPROGLUPHEMETGLU
2   GLYVALLEUTYRLYSTRPTHRASNTYRLEU
3   THRGLYTRPGLNPROARGTRPPHEVALLEU
4   ASPASNGLYILELEUSERTYRTYRASPSER
5   GLNASPASPVALCYSLYSGLYSERLYSGLY
6   SERILELYSMETALAVALCYSGLUILELYS
7   VALHISSERALAASPASNTHRARGMETGLU
8   LEUILEILEPROGLYGLUGLNHISPHETYR
9   METLYSALAVALASNALAALAGLUARGGLN
10   ARGTRPLEUVALALALEUGLYSERSERLYS
11   ALASERLEUTHRASPTHRARGTHR

Samples:

sample_1: FAPP1-PH, [U-13C; U-15N], 600 uM; TRIS 20 mM; sodium azide 1 mM; sodium chloride 100 mM

sample_2: FAPP1-PH, [U-15N], 600 uM; TRIS 20 mM; sodium azide 1 mM; sodium chloride 100 mM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

ARIA v2, Linge, O, . - structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

BMRB 19508 19576
PDB
DBJ BAG37067
GB AAG15199 AAG15200 AAH31110 AAH44567 AAH83759
REF NP_001013095 NP_001095383 NP_001230393 NP_061964 NP_112546
SP Q9ERS4 Q9HB20
TPG DAA32821

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts