BMRB Entry 16236
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16236
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Title: Bruno RRM3+ PubMed: 19919093
Deposition date: 2009-04-01 Original release date: 2009-11-23
Authors: Lyon, Angeline; Reveal, Brad; Macdonald, Paul; Hoffman, David
Citation: Lyon, Angeline; Reveal, Brad; Macdonald, Paul; Hoffman, David. "Bruno protein contains an expanded RNA recognition motif" Biochemistry 48, 12202-12212 (2009).
Assembly members:
Bruno_RRM3+, polymer, 118 residues, 12296.124 Da.
Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Bruno_RRM3+: AAGLPQFGSASALSTSPLAS
VALSAAAAAAAGKQIEGPEG
CNLFIYHLPQEFTDTDLAST
FLPFGNVISAKVFIDKQTSL
SKCFGFVSFDNPDSAQVAIK
AMNGFQVGTKRLKVQLKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 434 |
| 15N chemical shifts | 117 |
| 1H chemical shifts | 782 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Bruno_RRM3+ | 1 |
Entities:
Entity 1, Bruno_RRM3+ 118 residues - 12296.124 Da.
| 1 | ALA | ALA | GLY | LEU | PRO | GLN | PHE | GLY | SER | ALA | ||||
| 2 | SER | ALA | LEU | SER | THR | SER | PRO | LEU | ALA | SER | ||||
| 3 | VAL | ALA | LEU | SER | ALA | ALA | ALA | ALA | ALA | ALA | ||||
| 4 | ALA | GLY | LYS | GLN | ILE | GLU | GLY | PRO | GLU | GLY | ||||
| 5 | CYS | ASN | LEU | PHE | ILE | TYR | HIS | LEU | PRO | GLN | ||||
| 6 | GLU | PHE | THR | ASP | THR | ASP | LEU | ALA | SER | THR | ||||
| 7 | PHE | LEU | PRO | PHE | GLY | ASN | VAL | ILE | SER | ALA | ||||
| 8 | LYS | VAL | PHE | ILE | ASP | LYS | GLN | THR | SER | LEU | ||||
| 9 | SER | LYS | CYS | PHE | GLY | PHE | VAL | SER | PHE | ASP | ||||
| 10 | ASN | PRO | ASP | SER | ALA | GLN | VAL | ALA | ILE | LYS | ||||
| 11 | ALA | MET | ASN | GLY | PHE | GLN | VAL | GLY | THR | LYS | ||||
| 12 | ARG | LEU | LYS | VAL | GLN | LEU | LYS | LYS |
Samples:
Bruno_RRM3+_1: 1,0 mM Bruno RRM3+, [U-100% 13C; U-100% 15N], 1 mM; potassium phosphate 1 mM; sodium chloride300 – 400 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 260 mM; pH: 6; pressure: 1.0 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-13C NOESY | Bruno_RRM3+_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | Bruno_RRM3+_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | Bruno_RRM3+_1 | isotropic | sample_conditions_1 |
Software:
CNS v1.1., Brunger, Adams, Clore, Gros, Nilges and Read - simulated annealing
NMR spectrometers:
- Varian Inova 500 MHz
Related Database Links:
| PDB | |
| GB | AAL49272 AAM50707 AAN10813 ACL86931 AET11743 |
| REF | NP_723740 XP_002015057 |
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