BMRB Entry 16305
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16305
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Title: 1H, 13C and 15N assignments of a camelid nanobody directed against human alpha-synuclein PubMed: 19763886
Deposition date: 2009-05-18 Original release date: 2009-10-14
Authors: Vuchelen, Anneleen; O'Day, Elizabeth; Hsu, Danny; De Genst, Erwin
Citation: Vuchelen, Anneleen; O'Day, Elizabeth; De Genst, Erwin; Pardon, Els; Wyns, Lode; Dumoulin, Mireille; Dobson, Chris; Christodoulou, John; Hsu, Danny. "1H, 13C and 15N assignments of a camelid nanobody directed against human alpha-synuclein" Biomol. NMR Assignments 3, 231-233 (2009).
Assembly members:
NbSyn2, polymer, 132 residues, 14440.9 Da.
Natural source: Common Name: even-toed ungulates Taxonomy ID: 9844 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Lama glama
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
NbSyn2: QGQLVESGGGSVQAGGSLRL
SCAASGIDSSSYCMGWFRQR
PGKEREGVARINGLGGVKTA
YADSVKDRFTISRDNAENTV
YLQMNSLKPEDTAIYYCAAK
FSPGYCGGSWSNFGYWGQGT
QVTVSSHHHHHH
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 432 |
15N chemical shifts | 130 |
1H chemical shifts | 703 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | NbSyn2 | 1 |
Entities:
Entity 1, NbSyn2 132 residues - 14440.9 Da.
1 | GLN | GLY | GLN | LEU | VAL | GLU | SER | GLY | GLY | GLY | ||||
2 | SER | VAL | GLN | ALA | GLY | GLY | SER | LEU | ARG | LEU | ||||
3 | SER | CYS | ALA | ALA | SER | GLY | ILE | ASP | SER | SER | ||||
4 | SER | TYR | CYS | MET | GLY | TRP | PHE | ARG | GLN | ARG | ||||
5 | PRO | GLY | LYS | GLU | ARG | GLU | GLY | VAL | ALA | ARG | ||||
6 | ILE | ASN | GLY | LEU | GLY | GLY | VAL | LYS | THR | ALA | ||||
7 | TYR | ALA | ASP | SER | VAL | LYS | ASP | ARG | PHE | THR | ||||
8 | ILE | SER | ARG | ASP | ASN | ALA | GLU | ASN | THR | VAL | ||||
9 | TYR | LEU | GLN | MET | ASN | SER | LEU | LYS | PRO | GLU | ||||
10 | ASP | THR | ALA | ILE | TYR | TYR | CYS | ALA | ALA | LYS | ||||
11 | PHE | SER | PRO | GLY | TYR | CYS | GLY | GLY | SER | TRP | ||||
12 | SER | ASN | PHE | GLY | TYR | TRP | GLY | GLN | GLY | THR | ||||
13 | GLN | VAL | THR | VAL | SER | SER | HIS | HIS | HIS | HIS | ||||
14 | HIS | HIS |
Samples:
sample_1: NbSyn2, [U-95% 13C; U-95% 15N], 300 uM; H2O 90%; D2O 10%; sodium acetate 10%
NbSyn2_conditions: ionic strength: 20 mM; pH: 4.8; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D HNCO | sample_1 | isotropic | NbSyn2_conditions |
3D NOESY HSQC | sample_1 | isotropic | NbSyn2_conditions |
3D TOCSY HSQC | sample_1 | isotropic | NbSyn2_conditions |
2D 1H-15N HSQC | sample_1 | isotropic | NbSyn2_conditions |
2D 1H-13C HSQC | sample_1 | isotropic | NbSyn2_conditions |
3D HCCH-TOCSY | sample_1 | isotropic | NbSyn2_conditions |
3D HNCA | sample_1 | isotropic | NbSyn2_conditions |
3D HNCACB | sample_1 | isotropic | NbSyn2_conditions |
3D CBCA(CO)NH | sample_1 | isotropic | NbSyn2_conditions |
Software:
SPARKY, Goddard - chemical shift assignment, peak picking
TOPSPIN, Bruker Biospin - processing
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
MARS, Jung, YS and Zweckstetter, M - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz
Related Database Links:
PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts