BMRB Entry 16322
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16322
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Extracellular CD147 (EMMPRIN): Isoforms -2 PubMed: 19500591
Deposition date: 2009-05-28 Original release date: 2009-06-23
Authors: Schelgel, Jennifer; Redzic, Jasmina; Porter, Christopher; Yurchenko, Vyacheslav; Bukrinsky, Michael; Armstrong, Geoffrey; Zhang, Fengli; Isern, Nancy; Degregori, James; Hodges, Robert; Eisenmesser, Elan
Citation: Schlegel, Jennifer; Redzic, Jasmina; Porter, Christopher; Yurchenko, Vyacheslav; Bukrinsky, Michael; Labeikovsky, Wladimir; Armstrong, Geoffrey; Zhang, Fengli; Isern, Nancy; DeGregori, James; Hodges, Robert; Eisenmesser, Elan Zohar. "Solution characterization of the extracellular region of CD147 and its interaction with its enzyme ligand cyclophilin A." J. Mol. Biol. 391, 518-535 (2009).
Assembly members:
CD147_22-214, polymer, 198 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
CD147_22-214: GSHMMAAGTVFTTVEDLGSK
ILLTCSLNDSATEVTGHRWL
KGGVVLKEDALPGQKTEFKV
DSDDQWGEYSCVFLPEPMGT
ANIQLHGPPRVKAVKSSEHI
NEGETAMLVCKSESVPPVTD
WAWYKITDSEDKALMNGSES
RFFVSSSQGRSELHIENLNM
EADPGQYRCNGTSSKGSDQA
IITLRVRSHLAALWPFLG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 356 |
| 15N chemical shifts | 182 |
| 1H chemical shifts | 182 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Extracellular CD147 Iso-2, trans | 1 |
| 2 | Extracellular CD147 Iso-2, cis | 1 |
Entities:
Entity 1, Extracellular CD147 Iso-2, trans 198 residues - Formula weight is not available
residues 207-214 exist in both cis and trans conformations
| 1 | GLY | SER | HIS | MET | MET | ALA | ALA | GLY | THR | VAL | ||||
| 2 | PHE | THR | THR | VAL | GLU | ASP | LEU | GLY | SER | LYS | ||||
| 3 | ILE | LEU | LEU | THR | CYS | SER | LEU | ASN | ASP | SER | ||||
| 4 | ALA | THR | GLU | VAL | THR | GLY | HIS | ARG | TRP | LEU | ||||
| 5 | LYS | GLY | GLY | VAL | VAL | LEU | LYS | GLU | ASP | ALA | ||||
| 6 | LEU | PRO | GLY | GLN | LYS | THR | GLU | PHE | LYS | VAL | ||||
| 7 | ASP | SER | ASP | ASP | GLN | TRP | GLY | GLU | TYR | SER | ||||
| 8 | CYS | VAL | PHE | LEU | PRO | GLU | PRO | MET | GLY | THR | ||||
| 9 | ALA | ASN | ILE | GLN | LEU | HIS | GLY | PRO | PRO | ARG | ||||
| 10 | VAL | LYS | ALA | VAL | LYS | SER | SER | GLU | HIS | ILE | ||||
| 11 | ASN | GLU | GLY | GLU | THR | ALA | MET | LEU | VAL | CYS | ||||
| 12 | LYS | SER | GLU | SER | VAL | PRO | PRO | VAL | THR | ASP | ||||
| 13 | TRP | ALA | TRP | TYR | LYS | ILE | THR | ASP | SER | GLU | ||||
| 14 | ASP | LYS | ALA | LEU | MET | ASN | GLY | SER | GLU | SER | ||||
| 15 | ARG | PHE | PHE | VAL | SER | SER | SER | GLN | GLY | ARG | ||||
| 16 | SER | GLU | LEU | HIS | ILE | GLU | ASN | LEU | ASN | MET | ||||
| 17 | GLU | ALA | ASP | PRO | GLY | GLN | TYR | ARG | CYS | ASN | ||||
| 18 | GLY | THR | SER | SER | LYS | GLY | SER | ASP | GLN | ALA | ||||
| 19 | ILE | ILE | THR | LEU | ARG | VAL | ARG | SER | HIS | LEU | ||||
| 20 | ALA | ALA | LEU | TRP | PRO | PHE | LEU | GLY |
Samples:
sample_1: sodium phosphate 50 mM; sodium chloride 150 mM; imidazole 50 mM; CD147 22-214, [U-99% 13C; U-99% 15N], 0.5 mM; H2O 93%; D2O 7%
sample_conditions_1: ionic strength: 0.232 M; pH: 6.5; pressure: 1 atm; temperature: 298.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMRJ, Varian - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CcpNMR, CCPN - chemical shift assignment
NMR spectrometers:
- Varian VNMRS 900 MHz
- Varian INOVA 800 MHz
- Varian INOVA 800 MHz
- Varian INOVA 600 MHz
- Varian INOVA 720 MHz
Related Database Links:
| PDB | |
| DBJ | BAA08109 BAB88938 BAC67167 BAC67168 BAC76828 |
| EMBL | CAA45716 CAH92402 |
| GB | AAA68936 AAA91084 AAB41120 AAC33279 AAD10704 |
| REF | NP_001128860 NP_001719 NP_940991 NP_940992 XP_005259676 |
| SP | P35613 Q865R3 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts