BMRB Entry 16341
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16341
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Title: The inactive form of the retroviral protease of the murine intracisternal particle, inMIA-14 PR PubMed: 19856131
Deposition date: 2009-06-09 Original release date: 2009-11-16
Authors: Motackova, Veronika; Kubickova, Monika; Kozisek, Milan; Grantz-Saskova, Klara; Svec, Martin; Zidek, Lukas; Sklenar, Vladimir
Citation: Motakova, Veronika; Kubikova, Monika; Kozisek, Milan; Sakova, Klara; Svec, Martin; Zidek, Luka; Sklena, Vladimir. "Backbone (1)H, (13)C, and (15)N NMR assignment for the inactive form of the retroviral protease of the murine intracisternal A-type particle, inMIA-14 PR." Biomol NMR Assign 3, 261-264 (2009).
Assembly members:
inMIA14-PR, polymer, 156 residues, Formula weight is not available
Natural source: Common Name: Murine endogenous retrovirus Taxonomy ID: 35275 Superkingdom: Viruses Kingdom: not available Genus/species: Betaretrovirus Murine endogenous retrovirus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
inMIA14-PR: LVVSLNDRPKLRLKINGKEF
EGILDTGADKSIISTHWWPK
AWPTTESSHSLQGLGYQSCP
TISSVALTWESSEGQQGKFI
PYVLPLPVNLWGRDIMQHLG
LILSNENAPSGGYSTKAKNI
MAKMGYKEGKGLGHQEQGRI
EPISPNGNQDRQGLGF
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 416 |
15N chemical shifts | 135 |
1H chemical shifts | 135 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | inMIA14-PR | 1 |
Entities:
Entity 1, inMIA14-PR 156 residues - Formula weight is not available
1 | LEU | VAL | VAL | SER | LEU | ASN | ASP | ARG | PRO | LYS | ||||
2 | LEU | ARG | LEU | LYS | ILE | ASN | GLY | LYS | GLU | PHE | ||||
3 | GLU | GLY | ILE | LEU | ASP | THR | GLY | ALA | ASP | LYS | ||||
4 | SER | ILE | ILE | SER | THR | HIS | TRP | TRP | PRO | LYS | ||||
5 | ALA | TRP | PRO | THR | THR | GLU | SER | SER | HIS | SER | ||||
6 | LEU | GLN | GLY | LEU | GLY | TYR | GLN | SER | CYS | PRO | ||||
7 | THR | ILE | SER | SER | VAL | ALA | LEU | THR | TRP | GLU | ||||
8 | SER | SER | GLU | GLY | GLN | GLN | GLY | LYS | PHE | ILE | ||||
9 | PRO | TYR | VAL | LEU | PRO | LEU | PRO | VAL | ASN | LEU | ||||
10 | TRP | GLY | ARG | ASP | ILE | MET | GLN | HIS | LEU | GLY | ||||
11 | LEU | ILE | LEU | SER | ASN | GLU | ASN | ALA | PRO | SER | ||||
12 | GLY | GLY | TYR | SER | THR | LYS | ALA | LYS | ASN | ILE | ||||
13 | MET | ALA | LYS | MET | GLY | TYR | LYS | GLU | GLY | LYS | ||||
14 | GLY | LEU | GLY | HIS | GLN | GLU | GLN | GLY | ARG | ILE | ||||
15 | GLU | PRO | ILE | SER | PRO | ASN | GLY | ASN | GLN | ASP | ||||
16 | ARG | GLN | GLY | LEU | GLY | PHE |
Samples:
sample_1: inMIA14-PR, [U-100% 13C; U-100% 15N; U-75% 2H], 0.3 mM; sodium chloride 50 mM; sodium azide 50 uM; phosphate buffer 20 mM
sample_conditions_1: ionic strength: 20 mM; pH: 7.4; pressure: 1 atm; temperature: 296 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
DBJ | BAC79171 |
EMBL | CRH48730 CRH55731 CRH56783 CRH57961 |
GB | AAC12789 AAC52922 |
REF | WP_057247684 WP_057256782 |
SP | P11365 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts