BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16607

Title: SOLUTION STRUCTURE OF THE AL-09 H87Y IMMUNOGLOBULIN LIGHT CHAIN VARIABLE DOMAIN

Deposition date: 2009-11-11 Original release date: 2010-05-13

Authors: Volkman, B.; Peterson, F.; Ramirez-Alvarado, M.; Baden, E.

Citation: Baden, E.; Peterson, F.; Owen, B.; Volkman, B.; Ramirez-Alvarado, M.. "TBA"  To be published ., .-..

Assembly members:
AL-09_H87Y, polymer, 110 residues, 12154.475 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
AL-09_H87Y: STDIQMTQSPSSLSASVGDR VTITCQASQDINNYLIWYQQ KPGQAPKLLIYDASTLETGV PSRFSGSGSGTEFTFTISSL QPEDLATYYCQQYDNLPYTF GQGTKLEIKR

Data sets:
Data typeCount
13C chemical shifts906
15N chemical shifts229
1H chemical shifts1489

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AL-09 H87Y_11
2AL-09 H87Y_21

Entities:

Entity 1, AL-09 H87Y_1 110 residues - 12154.475 Da.

Numbering in the second monomer, chain B, is offset by 200. The ST dipeptide at the N-terminus is a cloning artifact.

1   SERTHRASPILEGLNMETTHRGLNSERPRO
2   SERSERLEUSERALASERVALGLYASPARG
3   VALTHRILETHRCYSGLNALASERGLNASP
4   ILEASNASNTYRLEUILETRPTYRGLNGLN
5   LYSPROGLYGLNALAPROLYSLEULEUILE
6   TYRASPALASERTHRLEUGLUTHRGLYVAL
7   PROSERARGPHESERGLYSERGLYSERGLY
8   THRGLUPHETHRPHETHRILESERSERLEU
9   GLNPROGLUASPLEUALATHRTYRTYRCYS
10   GLNGLNTYRASPASNLEUPROTYRTHRPHE
11   GLYGLNGLYTHRLYSLEUGLUILELYSARG

Samples:

sample_1: AL-09 H87Y, [U-100% 13C; U-100% 15N], 0.8 mM; MES, [U-2H], 10 mM; sodium azide 0.02%; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 8 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D_15N-separated_NOESYsample_1isotropicsample_conditions_1
3D_13C-separated_NOESYsample_1isotropicsample_conditions_1
3D_13C-separated_NOESY (AROMATIC)sample_1isotropicsample_conditions_1

Software:

X-PLOR NIH v2.9.3, SCHWIETERS,C.D.,KUSZEWSKI,J.J.,TJANDRA,N.,CLORE,G.M. - refinement

TOPSPIN v2.1, Bruker - collection

NMRPipe v2007, Delagio,F. et al. - processing

XEASY v1.3, Eccles, C., Guntert, P., Billeter, M., Wuthrich, K. - data analysis

GARANT v2.1, C. Bartels - data analysis

CYANA v2.1, Guntert, P. - structural calculation

NMR spectrometers:

  • Bruker Avance III 500 MHz

Related Database Links:

PDB
GB AAO11844

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts