BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17170

Title: Resonance assignments of the Myb2 DNA binding domain in complex with its promoter MRE-1 from Trichomonas vaginalis

Deposition date: 2010-09-07 Original release date: 2012-08-01

Authors: Huang, Tai-Huang; Jiang, Ingjye; Tsai, Chen-Kun; Naik, Mandar; Chang, Chi-Fon; Wu, Wen-Jin

Citation: Huang, Tai-huang; Jiang, Ingjye; Tsai, Chen-Kun. "Resonance assignments of the Myb2 DNA binding domain in complex with its promoter MRE-1 from Trichomonas vaginalis"  Not known ., .-..

Assembly members:
tvMyb2_MRE-1, polymer, 126 residues, Formula weight is not available

Natural source:   Common Name: Trichomonas vaginalis   Taxonomy ID: 5722   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Trichomonas vaginalis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
tvMyb2_MRE-1: MVQVNLKAAKKQKFTPEEDE MLKRAVAQHGSDWKMIAATF PNRNARQCRDRWKNYLAPSI SHTPWTAEEDALLVQKIQEY GRQWAIIAKFFPGRTDIHIK NRWVTISNKLGIPQTQQMLE HHHHHH

Data sets:
Data typeCount
13C chemical shifts476
15N chemical shifts112
1H chemical shifts723

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1tvMyb21

Entities:

Entity 1, tvMyb2 126 residues - Formula weight is not available

1   METVALGLNVALASNLEULYSALAALALYS
2   LYSGLNLYSPHETHRPROGLUGLUASPGLU
3   METLEULYSARGALAVALALAGLNHISGLY
4   SERASPTRPLYSMETILEALAALATHRPHE
5   PROASNARGASNALAARGGLNCYSARGASP
6   ARGTRPLYSASNTYRLEUALAPROSERILE
7   SERHISTHRPROTRPTHRALAGLUGLUASP
8   ALALEULEUVALGLNLYSILEGLNGLUTYR
9   GLYARGGLNTRPALAILEILEALALYSPHE
10   PHEPROGLYARGTHRASPILEHISILELYS
11   ASNARGTRPVALTHRILESERASNLYSLEU
12   GLYILEPROGLNTHRGLNGLNMETLEUGLU
13   HISHISHISHISHISHIS

Samples:

sample_1: EDTA 1 mM; DTT 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM; H2O 93%; D2O 7%; tvMyb2_MRE-1, [U-100% 13C; U-100% 15N], 0.6 mM

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - processing

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
GB AAX51242 EAY06671
REF XP_001318894

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts