BMRB Entry 17190
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17190
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Title: Complex Pfu Rpp30 PubMed: 22162665
Deposition date: 2010-09-13 Original release date: 2012-05-22
Authors: Crowe, Brandon; Foster, Mark
Citation: Crowe, Brandon; Bohlen, Christopher; Wilson, Ross; Gopalan, Venkat; Foster, Mark. "Assembly of the complex between archaeal RNase P proteins RPP30 and Pop5." Archaea 2011, 891531-891531 (2011).
Assembly members:
Rpp30, polymer, 217 residues, Formula weight is not available
Pop5, polymer, 120 residues, Formula weight is not available
Natural source: Common Name: Pyrococcus furiosus Taxonomy ID: 2261 Superkingdom: Archaea Kingdom: not available Genus/species: Pyrococcus furiosus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Rpp30: GEFMAGGRNGVKFVEMDIRS
REAYELAEEWFDDVVFSYEI
PPGVLDKERLKEIKKEYGNV
AITLINPKPSLVKEAVQRFK
QNYLIYVESSDLRVVRYSIE
RGVDAVISPWANRKDQGIDH
VLARMMNKRGVALGFSLRPL
LHQNPYERANALKFMRKAWT
LVNKYKVPRFISSSAKGKFQ
VRGVKELISLGIAIGMEEVQ
AKASLSFYPLGILERLK
Pop5: MSERPKTLPPTLRDKKRYIA
FKVISENQFNKDEIKEAIWN
ACLRTLGELGTAKAKPWLIK
FDETTQTGIIRSDRNHVYDV
IFSLTLVSDINGNKAIIKVL
GVSGTIKRLKRKFLSQFGWR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 465 |
| 15N chemical shifts | 180 |
| 1H chemical shifts | 180 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Rpp30 | 1 |
| 2 | Pop5 | 2 |
Entities:
Entity 1, Rpp30 217 residues - Formula weight is not available
Pfu Rpp30 native with 3 residues as artifact from cleavage of affinity tag.
| 1 | GLY | GLU | PHE | MET | ALA | GLY | GLY | ARG | ASN | GLY | ||||
| 2 | VAL | LYS | PHE | VAL | GLU | MET | ASP | ILE | ARG | SER | ||||
| 3 | ARG | GLU | ALA | TYR | GLU | LEU | ALA | GLU | GLU | TRP | ||||
| 4 | PHE | ASP | ASP | VAL | VAL | PHE | SER | TYR | GLU | ILE | ||||
| 5 | PRO | PRO | GLY | VAL | LEU | ASP | LYS | GLU | ARG | LEU | ||||
| 6 | LYS | GLU | ILE | LYS | LYS | GLU | TYR | GLY | ASN | VAL | ||||
| 7 | ALA | ILE | THR | LEU | ILE | ASN | PRO | LYS | PRO | SER | ||||
| 8 | LEU | VAL | LYS | GLU | ALA | VAL | GLN | ARG | PHE | LYS | ||||
| 9 | GLN | ASN | TYR | LEU | ILE | TYR | VAL | GLU | SER | SER | ||||
| 10 | ASP | LEU | ARG | VAL | VAL | ARG | TYR | SER | ILE | GLU | ||||
| 11 | ARG | GLY | VAL | ASP | ALA | VAL | ILE | SER | PRO | TRP | ||||
| 12 | ALA | ASN | ARG | LYS | ASP | GLN | GLY | ILE | ASP | HIS | ||||
| 13 | VAL | LEU | ALA | ARG | MET | MET | ASN | LYS | ARG | GLY | ||||
| 14 | VAL | ALA | LEU | GLY | PHE | SER | LEU | ARG | PRO | LEU | ||||
| 15 | LEU | HIS | GLN | ASN | PRO | TYR | GLU | ARG | ALA | ASN | ||||
| 16 | ALA | LEU | LYS | PHE | MET | ARG | LYS | ALA | TRP | THR | ||||
| 17 | LEU | VAL | ASN | LYS | TYR | LYS | VAL | PRO | ARG | PHE | ||||
| 18 | ILE | SER | SER | SER | ALA | LYS | GLY | LYS | PHE | GLN | ||||
| 19 | VAL | ARG | GLY | VAL | LYS | GLU | LEU | ILE | SER | LEU | ||||
| 20 | GLY | ILE | ALA | ILE | GLY | MET | GLU | GLU | VAL | GLN | ||||
| 21 | ALA | LYS | ALA | SER | LEU | SER | PHE | TYR | PRO | LEU | ||||
| 22 | GLY | ILE | LEU | GLU | ARG | LEU | LYS |
Entity 2, Pop5 120 residues - Formula weight is not available
Pop5 from Pfu with C72S mutation
| 1 | MET | SER | GLU | ARG | PRO | LYS | THR | LEU | PRO | PRO | |
| 2 | THR | LEU | ARG | ASP | LYS | LYS | ARG | TYR | ILE | ALA | |
| 3 | PHE | LYS | VAL | ILE | SER | GLU | ASN | GLN | PHE | ASN | |
| 4 | LYS | ASP | GLU | ILE | LYS | GLU | ALA | ILE | TRP | ASN | |
| 5 | ALA | CYS | LEU | ARG | THR | LEU | GLY | GLU | LEU | GLY | |
| 6 | THR | ALA | LYS | ALA | LYS | PRO | TRP | LEU | ILE | LYS | |
| 7 | PHE | ASP | GLU | THR | THR | GLN | THR | GLY | ILE | ILE | |
| 8 | ARG | SER | ASP | ARG | ASN | HIS | VAL | TYR | ASP | VAL | |
| 9 | ILE | PHE | SER | LEU | THR | LEU | VAL | SER | ASP | ILE | |
| 10 | ASN | GLY | ASN | LYS | ALA | ILE | ILE | LYS | VAL | LEU | |
| 11 | GLY | VAL | SER | GLY | THR | ILE | LYS | ARG | LEU | LYS | |
| 12 | ARG | LYS | PHE | LEU | SER | GLN | PHE | GLY | TRP | ARG |
Samples:
sample_1: Rpp30, [U-100% 13C; U-100% 15N], 450 uM; Pop5 450 uM; DSS 66.67 uM; D2O 5%; H2O 95%; TRIS 10 mM; Sodium Azide 0.02%
sample_conditions_1: pH: 4.0; pressure: 1 atm; temperature: 328 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
xwinnmr, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| BMRB | 17189 6917 |
| GB | AAL82038 AFN04726 AAL81502 AFN04159 |
| REF | WP_011013054 NP_579107 WP_011012525 YP_006492451 |
| SP | Q8TZS0 Q8U151 |
| PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts