BMRB Entry 17345
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17345
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Title: Solution NMR structure of human NFATC2IP ubiquitin-like domain, NFATC2IP_244_338, NESG target HT65A/OCSP target hs00387_244_338/SGC-toronto
Deposition date: 2010-12-03 Original release date: 2011-01-05
Authors: Wu, Bin; Yee, Adelinda; Houliston, Scott; Doherty, Ryan; Semesi, Anthony; Dhe-Paganon, Sirano; Arrowsmith, Cheryl
Citation: Doherty, Ryan; Wu, Bin; Yee, Adelinda; Lemak, Alexander; Houliston, Scott; Fares, Christophe; Srisalam, Sampath; Bulter, C; Edwards, Aled; Weigelt, Johan; Dhe-Paganon, Sirano; Arrowsmith, Cheryl. "Human NFATC2IP ubiquitin-like domains" Not known ., .-..
Assembly members:
NFATC2IP_244_338, polymer, 95 residues, 10396.201 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
NFATC2IP_244_338: QGQEDEVVLVEGPTLPETPR
LFPLKIRCRADLVRLPLRMS
EPLQSVVDHMATHLGVSPSR
ILLLFGETELSPTATPRTLK
LGVADIIDCVVLTSS
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 414 |
| 15N chemical shifts | 87 |
| 1H chemical shifts | 693 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NFATC2IP_244_338 | 1 |
Entities:
Entity 1, NFATC2IP_244_338 95 residues - 10396.201 Da.
| 1 | GLN | GLY | GLN | GLU | ASP | GLU | VAL | VAL | LEU | VAL | ||||
| 2 | GLU | GLY | PRO | THR | LEU | PRO | GLU | THR | PRO | ARG | ||||
| 3 | LEU | PHE | PRO | LEU | LYS | ILE | ARG | CYS | ARG | ALA | ||||
| 4 | ASP | LEU | VAL | ARG | LEU | PRO | LEU | ARG | MET | SER | ||||
| 5 | GLU | PRO | LEU | GLN | SER | VAL | VAL | ASP | HIS | MET | ||||
| 6 | ALA | THR | HIS | LEU | GLY | VAL | SER | PRO | SER | ARG | ||||
| 7 | ILE | LEU | LEU | LEU | PHE | GLY | GLU | THR | GLU | LEU | ||||
| 8 | SER | PRO | THR | ALA | THR | PRO | ARG | THR | LEU | LYS | ||||
| 9 | LEU | GLY | VAL | ALA | ASP | ILE | ILE | ASP | CYS | VAL | ||||
| 10 | VAL | LEU | THR | SER | SER |
Samples:
sample_1: NFATC2IP_244_338, [U-100% 13C; U-100% 15N], 0.5 mM; TRIS, [U-100% 2H], 10 mM; sodium chloride 300 mM; zinc sulphate 10 uM; DTT, [U-100% 2H], 10 mM; NaN3 0.01%; benzamidine 10 mM; inhibitor cocktail 1 x; H2O 90%; D2O 10%
sample_2: NFATC2IP_244_338, [U-100% 13C; U-100% 15N], 0.5 mM; TRIS, [U-100% 2H], 10 mM; sodium chloride 300 mM; zinc sulphate 10 uM; DTT, [U-100% 2H], 10 mM; NaN3 0.01%; benzamidine 10 mM; inhibitor cocktail 1 x; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 300 mM; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRPipe v2.3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
MDDGUI v1.0, Gutmanas, Arrowsmith - processing
SPARKY v3.95, Goddard - data analysis
FMCGUI v2.4, Lemak, Arrowsmith - chemical shift assignment
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
AutoStruct, Huang, Tejero, Powers and Montelione - NMR structure quality assessment
PSVS, Bhattacharya and Montelione - NMR structure quality assessment
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAC11189 |
| GB | AAH18311 AAH21551 AAH80628 AAI01742 AAI12183 |
| REF | NP_116204 XP_001146533 XP_002826318 XP_003804645 XP_004057462 |
| SP | Q8NCF5 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts