BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17435

Title: solution structure of the C-terminal domain of Salmonella H-NS   PubMed: 21673140

Deposition date: 2011-01-29 Original release date: 2015-06-23

Authors: Li, Yifei

Citation: Gordon, Blair; Li, Yifei; Cote, Atina; Weirauch, Matthew; Ding, Pengfei; Hughes, Timothy; Navarre, William; Xia, Bin; Liu, Jun. "Structural basis for recognition of AT-rich DNA by unrelated xenogeneic silencing proteins"  Proc. Natl. Acad. Sci. U. S. A. 108, 10690-10695 (2011).

Assembly members:
H-NS, polymer, 49 residues, 5564.294 Da.

Natural source:   Common Name: Salmonella typhimurium   Taxonomy ID: 90371   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Salmonella typhimurium

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
H-NS: AARPAKYSYVDENGETKTWT GQGRTPAVIKKAMEEQGKQL EDFLIKELE

Data sets:
Data typeCount
13C chemical shifts173
15N chemical shifts51
1H chemical shifts350

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1H-NS1

Entities:

Entity 1, H-NS 49 residues - 5564.294 Da.

1   ALAALAARGPROALALYSTYRSERTYRVAL
2   ASPGLUASNGLYGLUTHRLYSTHRTRPTHR
3   GLYGLNGLYARGTHRPROALAVALILELYS
4   LYSALAMETGLUGLUGLNGLYLYSGLNLEU
5   GLUASPPHELEUILELYSGLULEUGLU

Samples:

sample_1: C-terminal domain of Salmonella H-NS, [U-13C, >90%; U-15 N, >90%], 0.7 mM; sodium phosphate, [U-100% 13C; U-100% 15N], 50 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - geometry optimization

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAJ36716 BAP07615 GAL42187 GAL43811 GAL48349
EMBL CAA32549 CAD08382 CAR32863 CAR37239 CAR59200
GB AAB61148 AAL20669 AAO69288 AAV77088 AAX65652
PIR AC0650
REF NP_455749 NP_460710 WP_001287382 WP_001287383 WP_001748450
SP P0A1S2 P0A1S3 Q5PCT5

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts