BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17451

Title: Central domain of RSV M2-1   PubMed: 21523439

Deposition date: 2011-02-09 Original release date: 2011-05-03

Authors: Dubosclard, Virginie; Blondot, Marie-Lise; Eleouet, Jean-Francois; Bontems, Francois; Sizun, Christina

Citation: Dubosclard, Virginie; Blondot, Marie-Lise; Eleouet, Jean-Francois; Bontems, Francois; Sizun, Christina. "1H, 13C, and 15N resonance assignment of the central domain of hRSV transcription antitermination factor M2-1."  Biomol. NMR Assignments 5, 237-239 (2011).

Assembly members:
M2-1, polymer, 121 residues, Formula weight is not available

Natural source:   Common Name: Human syncytial respiratory virus   Taxonomy ID: 11245   Superkingdom: virus   Kingdom: not available   Genus/species: Pneumovirus Human respiratory syncytial virus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
M2-1: GSEISGAAELDRTEEYALGV VGVLESYIGSINNITKQSAC VAMSKLLTELNSDDIKKLRD NEEPNSPKIRVYNTVISYIE SNRKNNKQTIHLLKRLPADV LKKTIKNTLDIHKSITINNP K

Data sets:
Data typeCount
13C chemical shifts533
15N chemical shifts129
1H chemical shifts889
residual dipolar couplings100

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1M2-11

Entities:

Entity 1, M2-1 121 residues - Formula weight is not available

The two first residues GS are left after cleavage of the N-terminal GST tag. The M2-1 sequence starts at Glu59 and ends with Lys177.

1   GLYSERGLUILESERGLYALAALAGLULEU
2   ASPARGTHRGLUGLUTYRALALEUGLYVAL
3   VALGLYVALLEUGLUSERTYRILEGLYSER
4   ILEASNASNILETHRLYSGLNSERALACYS
5   VALALAMETSERLYSLEULEUTHRGLULEU
6   ASNSERASPASPILELYSLYSLEUARGASP
7   ASNGLUGLUPROASNSERPROLYSILEARG
8   VALTYRASNTHRVALILESERTYRILEGLU
9   SERASNARGLYSASNASNLYSGLNTHRILE
10   HISLEULEULYSARGLEUPROALAASPVAL
11   LEULYSLYSTHRILELYSASNTHRLEUASP
12   ILEHISLYSSERILETHRILEASNASNPRO
13   LYS

Samples:

M21-15N13C: M2-1, [U-100% 13C; U-100% 15N], 0.13 mM; sodium phosphate 50 mM; sodium chloride 150 mM; DTT 1 mM; H2O 93%; D2O 7%

M21-13C: M2-1, [U-100% 13C], 0.1 mM; sodium phosphate 50 mM; sodium chloride 150 mM; DTT 1 mM; D2O 100%

M21-15N-c12e5: M2-1, [U-100% 15N], 0.2 mM; sodium phosphate 50 mM; sodium chloride 150 mM; DTT 1 mM; c12e5 0.055 v/v; Hexanol 0.017 v/v; H2O 93%; D2O 7%

M21-15N-gel: M2-1, [U-100% 15N], 0.12 mM; sodium phosphate 50 mM; sodium chloride 150 mM; DTT 1 mM; acrylamide/bisacryamide 6%; H2O 93%; D2O 7%

M21-15N: M2-1, [U-100% 15N], 0.12 mM; sodium phosphate 50 mM; sodium chloride 150 mM; DTT 1 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 150 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCM21-15N13Cisotropicsample_conditions_1
3D HNCOM21-15N13Cisotropicsample_conditions_1
3D HNCAM21-15N13Cisotropicsample_conditions_1
3D HN(CO)CAM21-15N13Cisotropicsample_conditions_1
3D HNCACBM21-15N13Cisotropicsample_conditions_1
3D CBCA(CO)NHM21-15N13Cisotropicsample_conditions_1
3D 1H-15N NOESYM21-15Nisotropicsample_conditions_1
2D 1H-13C HSQCM21-13Cisotropicsample_conditions_1
3D HCCH-TOCSYM21-13Cisotropicsample_conditions_1
3D HCCH-TOCSY aromaticM21-13Cisotropicsample_conditions_1
3D CCH-TOCSYM21-13Cisotropicsample_conditions_1
3D 1H-13C NOESYM21-13Cisotropicsample_conditions_1
2D HBCBCGCDHDM21-13Cisotropicsample_conditions_1
2D 1H-15N IPAP HSQCM21-15N-c12e5anisotropicsample_conditions_1
2D 1H-15N IPAP HSQCM21-15N-gelanisotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 950 MHz

Related Database Links:

PDB
DBJ BAA00106
GB AAB59860 AAB86665 AAB86677 AAC14903 AAC55971
REF NP_044597
SP P04545

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts