BMRB Entry 17487
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17487
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for ICL3 of 5TH1A receptor PubMed: 21640070
Deposition date: 2011-02-24 Original release date: 2011-02-24
Authors: Kim, Young Mee; Gayen, shovanlal; Chen, Angela; Huang, Qiwei; kang, congbao
Citation: Chen, Angela Shuyi; Kim, Young Mee; Gayen, Shovanlal; Huang, Qiwei; Raida, Manfred; Kang, Congbao. "NMR structural study of the intracellular loop 3 of the serotonin 5-HT(1A) receptor and its interaction with calmodulin." Biochim. Biophys. Acta 1808, 2224-2232 (2011).
Assembly members:
ICL3_of_5-HT1A_receptor, polymer, 147 residues, 16043.0 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ICL3_of_5-HT1A_receptor: MGHHHHHHHHHHSSGHIEGR
HMAARFRIRKTVKKVEKTGA
DTRHGASPAPQPKKSVNGES
GSRNWRLGVESKAGGALCAN
GAVRQGDDGAALEVIEVHRV
GNSKEHLPLPSEAGPTPCAP
ASFERKNERNAEAKRKMALA
RERKTVK
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 351 |
15N chemical shifts | 111 |
1H chemical shifts | 211 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | ICL3 of 5HT1A receptor | 1 |
Entities:
Entity 1, ICL3 of 5HT1A receptor 147 residues - 16043.0 Da.
1 | MET | GLY | HIS | HIS | HIS | HIS | HIS | HIS | HIS | HIS | ||||
2 | HIS | HIS | SER | SER | GLY | HIS | ILE | GLU | GLY | ARG | ||||
3 | HIS | MET | ALA | ALA | ARG | PHE | ARG | ILE | ARG | LYS | ||||
4 | THR | VAL | LYS | LYS | VAL | GLU | LYS | THR | GLY | ALA | ||||
5 | ASP | THR | ARG | HIS | GLY | ALA | SER | PRO | ALA | PRO | ||||
6 | GLN | PRO | LYS | LYS | SER | VAL | ASN | GLY | GLU | SER | ||||
7 | GLY | SER | ARG | ASN | TRP | ARG | LEU | GLY | VAL | GLU | ||||
8 | SER | LYS | ALA | GLY | GLY | ALA | LEU | CYS | ALA | ASN | ||||
9 | GLY | ALA | VAL | ARG | GLN | GLY | ASP | ASP | GLY | ALA | ||||
10 | ALA | LEU | GLU | VAL | ILE | GLU | VAL | HIS | ARG | VAL | ||||
11 | GLY | ASN | SER | LYS | GLU | HIS | LEU | PRO | LEU | PRO | ||||
12 | SER | GLU | ALA | GLY | PRO | THR | PRO | CYS | ALA | PRO | ||||
13 | ALA | SER | PHE | GLU | ARG | LYS | ASN | GLU | ARG | ASN | ||||
14 | ALA | GLU | ALA | LYS | ARG | LYS | MET | ALA | LEU | ALA | ||||
15 | ARG | GLU | ARG | LYS | THR | VAL | LYS |
Samples:
sample_1: ICL3 of 5-HT1A, [U-99% 13C; U-99% 15N], 0.5 mM; D2O, [U-99% 2H], 10%; sodium phosphate 20 mM; sodium chloride 150 mM; EDTA 2 mM; H2O 90%; DTT 1 mM
sample_2: ICL3 of 5-HT1A, [U-100% 15N], 0.5 mM; D2O, [U-99% 2H], 10%; sodium phosphate 20 mM; sodium chloride 150 mM; DTT 1 mM; EDTA 2 mM; H2O 90%
sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HCACO | sample_1 | isotropic | sample_conditions_1 |
3D HNHA | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Bruker Biospin, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard - chemical shift assignment, collection, processing
NMR spectrometers:
- Bruker Avance 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts