BMRB Entry 17491

Name: Backbone Resonance Assignments for the C-terminus of Prp24
Published: 2011-06-22

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17491

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone Resonance Assignments for the C-terminus of Prp24 PubMed: 21653550

Deposition date: 2011-02-25 Original release date: 2011-06-22

Authors: Martin-Tumasz, Stephen; Butcher, Samuel

Citation: Martin-Tumasz, Stephen; Richie, Ashley; Clos, Lawrence; Brow, David; Butcher, Samuel. "A novel occluded RNA recognition motif in Prp24 unwinds the U6 RNA internal stem loop." Nucleic Acids Res. 39, 7837-7847 (2011).

Assembly members:
L4C, polymer, 162 residues, 12568.729 Da.

Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
L4C: MFLERNEVKRLLASRNSKEL ETLICLFPLSDKVSPSLICQ FLQEEIHINEKDIRKILLVS DFNGAIIIFRDSKFAAKMLM ILNGSQFQGKVIRSGTINDM KRYYNNQQNHSMKHVKPSCI NMMEKGPNLQVKKKIPDKQE QMSNDDFRKMFLGELEHHHH HH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	389
15N chemical shifts	126
1H chemical shifts	472

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	L4C	1

Entities:

Entity 1, L4C 162 residues - 12568.729 Da.

1

MET

PHE

LEU

GLU

ARG

ASN

GLU

VAL

LYS

ARG

2

LEU

ALA

SER

ARG

ASN

SER

LYS

GLU

LEU

3

GLU

THR

LEU

ILE

CYS

LEU

PHE

PRO

LEU

SER

4

ASP

LYS

VAL

SER

PRO

SER

LEU

ILE

CYS

GLN

5

PHE

LEU

GLN

GLU

ILE

HIS

ILE

ASN

GLU

6

LYS

ASP

ILE

ARG

LYS

ILE

LEU

VAL

SER

7

ASP

PHE

ASN

GLY

ALA

ILE

PHE

ARG

8

ASP

SER

LYS

PHE

ALA

LYS

MET

LEU

MET

9

ILE

LEU

ASN

GLY

SER

GLN

PHE

GLN

GLY

LYS

10

VAL

ILE

ARG

SER

GLY

THR

ILE

ASN

ASP

MET

11

LYS

ARG

TYR

ASN

GLN

ASN

HIS

12

SER

MET

LYS

HIS

VAL

LYS

PRO

SER

CYS

ILE

13

ASN

MET

GLU

LYS

GLY

PRO

ASN

LEU

GLN

14

VAL

LYS

ILE

PRO

ASP

LYS

GLN

GLU

15

GLN

MET

SER

ASN

ASP

PHE

ARG

LYS

MET

16

PHE

LEU

GLY

GLU

LEU

GLU

HIS

17

HIS

Samples:

13C15N_H2O: L4C, [U-99% 13C; U-99% 15N], 600 uM; potassium phosphate pH 6 18 mM; potassium chloride 45 mM; DTT 0.9 mM; D2O 10%; H2O 90%

Reference: L4C, [U-99% 13C; U-99% 15N], 600 uM; potassium phosphate pH 6 18 mM; potassium chloride 45 mM; DSS 1 uM; D2O 10%; H2O 90%

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	13C15N_H2O	isotropic	sample_conditions_1
3D CBCA(CO)NH	13C15N_H2O	isotropic	sample_conditions_1
3D HNCACB	13C15N_H2O	isotropic	sample_conditions_1
3D HNCO	13C15N_H2O	isotropic	sample_conditions_1
3D HBHA(CO)NH	13C15N_H2O	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	Reference	isotropic	sample_conditions_1

Software:

VNMRJ, Varian - collection

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

Varian Uniform NMR System 900 MHz

BMRB	17490
PDB	2L9W 4N0T
EMBL	CAA89251 CAY82100
GB	AAU09775 AHY76723 AJP40962 AJS62134 AJS62569
REF	NP_013995
SP	P49960
TPG	DAA10168

Biological Magnetic Resonance Data Bank