BMRB Entry 17497
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17497
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Title: SNX-17-PX-DOMAIN PubMed: 21512128
Deposition date: 2011-02-28 Original release date: 2011-05-19
Authors: Mobli, Mehdi; Ghai, Rajesh
Citation: Ghai, Rajesh; Mobli, Mehdi; Norwood, Suzanne; Bugarcic, Andrea; Teasdale, Rohan; King, Glenn; Collins, Brett. "Phox homology band 4.1/ezrin/radixin/moesin-like proteins function as molecular scaffolds that interact with cargo receptors and Ras GTPases." Proc. Natl. Acad. Sci. U.S.A. 108, 7763-7768 (2011).
Assembly members:
SNX17-PX, polymer, 112 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
SNX17-PX: NAMHFSIPETESRSGDSGGS
AYVAYNIHVNGVLHCRVRYS
QLLGLHEQLRKEYGANVLPA
FPPKKLFSLTPAEVEQRREQ
LEKYMQAVRQDPLLGSSETF
NSFLRRAQQETQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 370 |
| 15N chemical shifts | 105 |
| 1H chemical shifts | 540 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SNX17-PX | 1 |
Entities:
Entity 1, SNX17-PX 112 residues - Formula weight is not available
| 1 | ASN | ALA | MET | HIS | PHE | SER | ILE | PRO | GLU | THR | ||||
| 2 | GLU | SER | ARG | SER | GLY | ASP | SER | GLY | GLY | SER | ||||
| 3 | ALA | TYR | VAL | ALA | TYR | ASN | ILE | HIS | VAL | ASN | ||||
| 4 | GLY | VAL | LEU | HIS | CYS | ARG | VAL | ARG | TYR | SER | ||||
| 5 | GLN | LEU | LEU | GLY | LEU | HIS | GLU | GLN | LEU | ARG | ||||
| 6 | LYS | GLU | TYR | GLY | ALA | ASN | VAL | LEU | PRO | ALA | ||||
| 7 | PHE | PRO | PRO | LYS | LYS | LEU | PHE | SER | LEU | THR | ||||
| 8 | PRO | ALA | GLU | VAL | GLU | GLN | ARG | ARG | GLU | GLN | ||||
| 9 | LEU | GLU | LYS | TYR | MET | GLN | ALA | VAL | ARG | GLN | ||||
| 10 | ASP | PRO | LEU | LEU | GLY | SER | SER | GLU | THR | PHE | ||||
| 11 | ASN | SER | PHE | LEU | ARG | ARG | ALA | GLN | GLN | GLU | ||||
| 12 | THR | GLN |
Samples:
sample_1: SNX17-PX, [U-100% 13C; U-100% 15N], 0.8 mM; D2O, [U-100% 2H], 5%; HEPES 10 mM; sodium chloride 100 mM; Lithium Sulphate 10 mM; DTT 2 mM; H2O, [U-100% 2H], 95%
sample_conditions_1: ionic strength: 100 mM; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
XEASY, Bartels et al. - chemical shift assignment
Rowland NMR Toolkit, J. C. Hoch & A. S. Stern - processing
NMR spectrometers:
- Bruker Avance 900 MHz
Related Database Links:
| PDB | |
| DBJ | BAA06542 BAC36927 BAC37004 BAD96263 BAE33663 |
| EMBL | CAC12897 CAG33362 CAH93411 |
| GB | AAH02524 AAH02610 AAH14620 AAH23732 AAH26571 |
| REF | NP_001011981 NP_001015638 NP_001127002 NP_001252904 NP_001253990 |
| SP | Q15036 Q5EA77 Q5R4A5 Q6AYS6 Q8BVL3 |
| TPG | DAA24411 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts