BMRB Entry 17505
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17505
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Title: 1H, 13C, and 15N Chemical Shift Assignments for human muscle Acylphosphatase PubMed: 21643968
Deposition date: 2011-03-03 Original release date: 2011-06-07
Authors: Fusco, Giuliana; Hsu, Shang-Te; Bemporad, Francesco; Vendruscolo, Michele; Chiti, Fabrizio; Dobson, Christopher
Citation: Fusco, Giuliana; De Simone, Alfonso; Hsu, Shang-Te Danny; Bemporad, Francesco; Vendruscolo, Michele; Chiti, Fabrizio; Dobson, Christopher. "(1)H, (13)C and (15)N resonance assignments of human muscle acylphosphatase." Biomol. NMR Assignments 6, 27-29 (2012).
Assembly members:
human_muscle_acylphosphatase_polypeptide, polymer, 99 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
human_muscle_acylphosphatase_polypeptide: MSTAQSLKSVDYEVFGRVQG
VSFRMYTEDEARKIGVVGWV
KNTSKGTVTGQVQGPEDKVN
SMKSWLSKVGSPSSRIDRTN
FSNEKTISKLEYSNFSIRY
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 386 |
| 15N chemical shifts | 103 |
| 1H chemical shifts | 588 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | human muscle acylphosphatase polypeptide | 1 |
Entities:
Entity 1, human muscle acylphosphatase polypeptide 99 residues - Formula weight is not available
| 1 | MET | SER | THR | ALA | GLN | SER | LEU | LYS | SER | VAL | ||||
| 2 | ASP | TYR | GLU | VAL | PHE | GLY | ARG | VAL | GLN | GLY | ||||
| 3 | VAL | SER | PHE | ARG | MET | TYR | THR | GLU | ASP | GLU | ||||
| 4 | ALA | ARG | LYS | ILE | GLY | VAL | VAL | GLY | TRP | VAL | ||||
| 5 | LYS | ASN | THR | SER | LYS | GLY | THR | VAL | THR | GLY | ||||
| 6 | GLN | VAL | GLN | GLY | PRO | GLU | ASP | LYS | VAL | ASN | ||||
| 7 | SER | MET | LYS | SER | TRP | LEU | SER | LYS | VAL | GLY | ||||
| 8 | SER | PRO | SER | SER | ARG | ILE | ASP | ARG | THR | ASN | ||||
| 9 | PHE | SER | ASN | GLU | LYS | THR | ILE | SER | LYS | LEU | ||||
| 10 | GLU | TYR | SER | ASN | PHE | SER | ILE | ARG | TYR |
Samples:
sample_1: human muscle acylphosphatase polypeptide, [U-95% 13C; U-95% 15N], 250 uM; sodium phosphate 50 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Bruker Biospin, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 500 MHz
Related Database Links:
| EMBL | CAA58988 |
| GB | AAH12290 AAY14721 AIC48219 EAX00154 |
| PRF | 2122227B |
| REF | NP_612457 XP_003262429 XP_003830881 XP_004029284 XP_009440741 |
| SP | P14621 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts