BMRB Entry 17511
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17511
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Leucine Zipper GCN4 coiled coil and x-form conformations. PubMed: 17469817
Deposition date: 2011-03-06 Original release date: 2011-04-04
Authors: Nikolaev, Yaroslav; Pervushin, Konstantin
Citation: Nikolaev, Yaroslav; Pervushin, Konstantin. "NMR spin state exchange spectroscopy reveals equilibrium of two distinct conformations of leucine zipper GCN4 in solution." J. Am. Chem. Soc. 129, 6461-6469 (2007).
Assembly members:
LZ-GCN4, polymer, 36 residues, Formula weight is not available
Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
LZ-GCN4: GLQRMKQLEDKVEELLSKNY
HLENEVARLKKLVGER
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 69 |
15N chemical shifts | 104 |
1H chemical shifts | 173 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | LZ-GCN4 dimer, chain 1 | 1 |
2 | LZ-GCN4 dimer, chain 2 | 1 |
3 | LZ-GCN4 monomer | 1 |
Entities:
Entity 1, LZ-GCN4 dimer, chain 1 36 residues - Formula weight is not available
First Glycine is a non-native residue from the expression system.
1 | GLY | LEU | GLN | ARG | MET | LYS | GLN | LEU | GLU | ASP | ||||
2 | LYS | VAL | GLU | GLU | LEU | LEU | SER | LYS | ASN | TYR | ||||
3 | HIS | LEU | GLU | ASN | GLU | VAL | ALA | ARG | LEU | LYS | ||||
4 | LYS | LEU | VAL | GLY | GLU | ARG |
Samples:
LZ-GCN4_NC: LZ-GCN4, [U-13C; U-15N], 0.3 mM; potassium chloride 80 mM; sodium acetate 40 mM
pH_3.2_310K: pH: 3.2; pressure: 1 atm; temperature: 310 K
pH_7.1_310K: pH: 7.1; temperature: 310 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D HNCACB | LZ-GCN4_NC | isotropic | pH_3.2_310K |
2D 1H-15N HSQC | LZ-GCN4_NC | isotropic | pH_7.1_310K |
3D 1H-15N NOESY | LZ-GCN4_NC | isotropic | pH_7.1_310K |
Software:
CARA, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker AMX 600 MHz
- Bruker AMX 900 MHz
Related Database Links:
BMRB | 1396 1397 1398 1399 1451 1452 1453 1454 15316 2062 371 |
PDB | |
DBJ | GAA22815 |
EMBL | CAE52206 CAE52207 CAE52208 CAE52209 CAE52210 |
GB | AAA34640 AAB64486 AAK07887 AAL09032 AHY75543 |
REF | NP_010907 |
SP | P03069 |
TPG | DAA07643 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts