BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17514

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Leucine Zipper c-Jun.   PubMed: 22374868

Deposition date: 2011-03-07 Original release date: 2012-08-07

Authors: Nikolaev, Yaroslav; Pervushin, Konstantin

Citation: Nikolaev, Yaroslav; Pervushin, Konstantin. "Structural basis of RNA binding by leucine zipper GCN4"  Protein Sci. 21, 667-676 (2012).

Assembly members:
LZ-cJun, polymer, 42 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
LZ-cJun: GLERIARLEEKVKTLKAQNS ELASTANMLREQVAQLKQKV MN

Data sets:
Data typeCount
13C chemical shifts78
15N chemical shifts40
1H chemical shifts40

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LZ-cJun, 11
2LZ-cJun, 21

Entities:

Entity 1, LZ-cJun, 1 42 residues - Formula weight is not available

1   GLYLEUGLUARGILEALAARGLEUGLUGLU
2   LYSVALLYSTHRLEULYSALAGLNASNSER
3   GLULEUALASERTHRALAASNMETLEUARG
4   GLUGLNVALALAGLNLEULYSGLNLYSVAL
5   METASN

Samples:

lzjun_NC: LZ-cJun, [U-13C; U-15N], 0.5 mM; KCl 85 mM

pH_7.1_295K: pH: 7.1; pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHlzjun_NCisotropicpH_7.1_295K
3D 1H-15N NOESYlzjun_NCisotropicpH_7.1_295K

Software:

CARA, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker AMX 800 MHz

Related Database Links:

BMRB 3394
PDB
DBJ BAD98539 BAD98540 BAD98541 BAE20944 BAE22389
EMBL CAA31236 CAA31252 CAA33553 CAA35041 CAA35084
GB AAA37419 AAA42416 AAA48927 AAA49537 AAA59197
PRF 1404381A 1411298A 1411300A 2107314A
REF NP_001026460 NP_001071295 NP_001185876 NP_001252779 NP_001274537
SP O77627 P05411 P05412 P05627 P12981
TPG DAA31168

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts