BMRB Entry 17514
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17514
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Leucine Zipper c-Jun. PubMed: 22374868
Deposition date: 2011-03-07 Original release date: 2012-08-07
Authors: Nikolaev, Yaroslav; Pervushin, Konstantin
Citation: Nikolaev, Yaroslav; Pervushin, Konstantin. "Structural basis of RNA binding by leucine zipper GCN4" Protein Sci. 21, 667-676 (2012).
Assembly members:
LZ-cJun, polymer, 42 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
LZ-cJun: GLERIARLEEKVKTLKAQNS
ELASTANMLREQVAQLKQKV
MN
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 78 |
15N chemical shifts | 40 |
1H chemical shifts | 40 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | LZ-cJun, 1 | 1 |
2 | LZ-cJun, 2 | 1 |
Entities:
Entity 1, LZ-cJun, 1 42 residues - Formula weight is not available
1 | GLY | LEU | GLU | ARG | ILE | ALA | ARG | LEU | GLU | GLU | ||||
2 | LYS | VAL | LYS | THR | LEU | LYS | ALA | GLN | ASN | SER | ||||
3 | GLU | LEU | ALA | SER | THR | ALA | ASN | MET | LEU | ARG | ||||
4 | GLU | GLN | VAL | ALA | GLN | LEU | LYS | GLN | LYS | VAL | ||||
5 | MET | ASN |
Samples:
lzjun_NC: LZ-cJun, [U-13C; U-15N], 0.5 mM; KCl 85 mM
pH_7.1_295K: pH: 7.1; pressure: 1 atm; temperature: 295 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D CBCA(CO)NH | lzjun_NC | isotropic | pH_7.1_295K |
3D 1H-15N NOESY | lzjun_NC | isotropic | pH_7.1_295K |
Software:
CARA, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking
TOPSPIN, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker AMX 800 MHz
Related Database Links:
BMRB | 3394 |
PDB | |
DBJ | BAD98539 BAD98540 BAD98541 BAE20944 BAE22389 |
EMBL | CAA31236 CAA31252 CAA33553 CAA35041 CAA35084 |
GB | AAA37419 AAA42416 AAA48927 AAA49537 AAA59197 |
PRF | 1404381A 1411298A 1411300A 2107314A |
REF | NP_001026460 NP_001071295 NP_001185876 NP_001252779 NP_001274537 |
SP | O77627 P05411 P05412 P05627 P12981 |
TPG | DAA31168 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts