BMRB Entry 17556
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17556
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Title: 1H, 13C, and 15N Chemical Shift Assignments of the yPEPmin fragment of Rsa1p PubMed: 24234454
Deposition date: 2011-03-30 Original release date: 2014-04-23
Authors: Quinternet, Marc; Back, Regis; Branlant, Christiane; Manival, Xavier
Citation: Rothe, Benjamin; Back, Regis; Quinternet, Marc; Bizarro, Jonathan; Robert, Marie-Cecile; Blaud, Magali; Romier, Christophe; Manival, Xavier; Charpentier, Bruno; Bertrand, Edouard; Branlant, Christiane. "Characterization of the interaction between protein Snu13p/15.5K and the Rsa1p/NUFIP factor and demonstration of its functional importance for snoRNP assembly." Nucleic Acids Res. 42, 2015-2036 (2014).
Assembly members:
yPEPmin_of_Rsa1p, polymer, 15 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
yPEPmin_of_Rsa1p: TDEDVKKWREERKKM
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 56 |
15N chemical shifts | 14 |
1H chemical shifts | 116 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | yPEPmin | 1 |
Entities:
Entity 1, yPEPmin 15 residues - Formula weight is not available
1 | THR | ASP | GLU | ASP | VAL | LYS | LYS | TRP | ARG | GLU | ||||
2 | GLU | ARG | LYS | LYS | MET |
Samples:
sample_1: yPEPmin of Rsa1p 3 ± 0.1 mM; sodium phosphate 10 mM; sodium chloride 150 mM; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 150 mM; pH: 6.4; pressure: 1 atm; temperature: 293 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA, Keller and Wuthrich - chemical shift assignment
CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts