BMRB Entry 17567
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17567
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Title: 26 nt artificial stemloop TASL2 PubMed: 22252483
Deposition date: 2011-04-01 Original release date: 2012-01-24
Authors: Aeschbacher, Thomas; Schubert, Mario; Allain, Frederic
Citation: Aeschbacher, Thomas; Schubert, Mario; Allain, Frederic H-T. "A procedure to validate and correct the (13)C chemical shift calibration of RNA datasets." J. Biomol. NMR 52, 179-190 (2012).
Assembly members:
TASL2, polymer, 26 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: cell free synthesis Host organism: none
Entity Sequences (FASTA):
TASL2: GGGAUUCAUGCUUCGGCAUG
AAUCCC
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 77 |
1H chemical shifts | 101 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | TASL2 | 1 |
Entities:
Entity 1, TASL2 26 residues - Formula weight is not available
1 | G | G | G | A | U | U | C | A | U | G | ||||
2 | C | U | U | C | G | G | C | A | U | G | ||||
3 | A | A | U | C | C | C |
Samples:
sample_1: TASL2 2 mM; D2O 100%
sample_2: TASL2 2 mM; H2O 95%; D2O 5%
sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 303 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 900 MHz