BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17635

Title: solution structure of FUS/TLS RRM domain

Deposition date: 2011-05-10 Original release date: 2012-06-04

Authors: Liu, Xuehui; Ren, Jintao; Niu, Chunyan; Gong, Weimin; Feng, Wei

Citation: Liu, Xuehui; Ren, Jintao; Niu, Chunyan; Feng, Wei; Gong, Weimin. "TLS-RRM is a promiscuous nucleic acid binding domain"  Not known ., .-..

Assembly members:
FUS/TLS RRM domain, polymer, 108 residues, 11747.013 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
FUS/TLS RRM domain: EQDNSDNNTIFVQGLGENVT IESVADYFKQIGIIKTNKKT GQPMINLYTDRETGKLKGEA TVSFDDPPSAKAAIDWFDGK EFSGNPIKVSFATRRADFNR GGGNGRGG

Data sets:
Data typeCount
13C chemical shifts446
15N chemical shifts117
1H chemical shifts657

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FUS/TLS RRM domain1

Entities:

Entity 1, FUS/TLS RRM domain 108 residues - 11747.013 Da.

1   GLUGLNASPASNSERASPASNASNTHRILE
2   PHEVALGLNGLYLEUGLYGLUASNVALTHR
3   ILEGLUSERVALALAASPTYRPHELYSGLN
4   ILEGLYILEILELYSTHRASNLYSLYSTHR
5   GLYGLNPROMETILEASNLEUTYRTHRASP
6   ARGGLUTHRGLYLYSLEULYSGLYGLUALA
7   THRVALSERPHEASPASPPROPROSERALA
8   LYSALAALAILEASPTRPPHEASPGLYLYS
9   GLUPHESERGLYASNPROILELYSVALSER
10   PHEALATHRARGARGALAASPPHEASNARG
11   GLYGLYGLYASNGLYARGGLYGLY

Samples:

sample_1: DSS 0.5 mM; Tris 20 mM; NaCl 50 mM; H2O 10%; D2O, [U-100% 2H], 90%; NaN3 0.02%; FUS_TLS_RRM_domain, [U-100% 15N; U-100% 13C], 1 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAD92139 BAE24196 BAE26524 BAE26972 BAE87186
EMBL CAA50559 CAG33028 CAJ83479
GB AAA79948 AAB27102 AAC13543 AAC35284 AAC35285
PRF 1916411B
REF NP_001001531 NP_001012137 NP_001080383 NP_001086293 NP_001090836
SP P35637 P56959 Q28009
TPG DAA15352

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts