BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17651

Title: Solution Structure of Histidine Phosphotransfer Domain of CheA   PubMed: 21947914

Deposition date: 2011-05-16 Original release date: 2011-10-12

Authors: Vu, Anh; Hamel, Damon; Zhou, Hongjun; Dahlquist, Frederick

Citation: Vu, Anh; Hamel, Damon; Zhou, Hongjun; Dahlquist, Frederick. "The structure and dynamic properties of the complete histidine phosphotransfer domain of the chemotaxis specific histidine autokinase CheA from Thermotoga maritima."  J. Biomol. NMR 51, 49-55 (2011).

Assembly members:
CheA, polymer, 133 residues, 14948.087 Da.

Natural source:   Common Name: Thermotoga maritima   Taxonomy ID: 2336   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermotoga maritima

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CheA: MMEEYLGVFVDETKEYLQNL NDTLLELEKNPEDMELINEA FRALHTLKGMAGTMGFSSMA KLCHTLENILDKARNSEIKI TSDLLDKIFAGVDMITRMVD KIVSEGSDDIGENIDVFSDT IKSFASSGKEKLE

Data sets:
Data typeCount
13C chemical shifts420
15N chemical shifts130
1H chemical shifts869

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CheA1

Entities:

Entity 1, CheA 133 residues - 14948.087 Da.

1   METMETGLUGLUTYRLEUGLYVALPHEVAL
2   ASPGLUTHRLYSGLUTYRLEUGLNASNLEU
3   ASNASPTHRLEULEUGLULEUGLULYSASN
4   PROGLUASPMETGLULEUILEASNGLUALA
5   PHEARGALALEUHISTHRLEULYSGLYMET
6   ALAGLYTHRMETGLYPHESERSERMETALA
7   LYSLEUCYSHISTHRLEUGLUASNILELEU
8   ASPLYSALAARGASNSERGLUILELYSILE
9   THRSERASPLEULEUASPLYSILEPHEALA
10   GLYVALASPMETILETHRARGMETVALASP
11   LYSILEVALSERGLUGLYSERASPASPILE
12   GLYGLUASNILEASPVALPHESERASPTHR
13   ILELYSSERPHEALASERSERGLYLYSGLU
14   LYSLEUGLU

Samples:

sample_1: H2O 90%; D2O, [U-99% 2H], 10%; P1, [U-99% 13C; U-99% 15N], 1 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
4D 15N,13C NOESYsample_1isotropicsample_conditions_1
4D 13C,13C NOESYsample_1isotropicsample_conditions_1

Software:

X-PLOR v3.851, Brunger - structure solution

ANSIG, Kraulis - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AAA96387 AAD35784 ABQ46257 ACB08586 ADA66595
REF NP_228511 WP_004081040 WP_008192691 WP_011942905 WP_012310403
SP Q56310

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts