BMRB Entry 17664
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17664
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Title: HVS ORF57 103-120 backbone assignment PubMed: 21253573
Deposition date: 2011-05-25 Original release date: 2011-09-01
Authors: Tunnicliffe, Richard; Hautbergue, Guillaume; Wilson, Stuart; Golovanov, Alexander
Citation: Tunnicliffe, Richard; Hautbergue, Guillaume; Kalra, Priti; Jackson, Brian; Whitehouse, Adrian; Wilson, Stuart; Golovanov, Alexander. "Structural basis for the recognition of cellular mRNA export factor REF by herpes viral proteins HSV-1 ICP27 and HVS ORF57." PLoS Pathog. 7, .-. (2011).
Assembly members:
HVS_ORF57, polymer, 23 residues, Formula weight is not available
Natural source: Common Name: Herpesvirus saimiri Taxonomy ID: 10381 Superkingdom: Viruses Kingdom: not available Genus/species: Herpesvirus saimiri
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
HVS_ORF57: GPLGSSCKTSWADRVREAAA
QRR
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 85 |
15N chemical shifts | 23 |
1H chemical shifts | 120 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | ORF57 103-120 | 1 |
Entities:
Entity 1, ORF57 103-120 23 residues - Formula weight is not available
N-terminal sequence GPLGS is non-native from cloning
1 | GLY | PRO | LEU | GLY | SER | SER | CYS | LYS | THR | SER | ||||
2 | TRP | ALA | ASP | ARG | VAL | ARG | GLU | ALA | ALA | ALA | ||||
3 | GLN | ARG | ARG |
Samples:
sample_1: HVS ORF57, [U-95% 13C; U-95% 15N], 1.0 ± 0.05 mM; H20 90%; D20 10%
sample_conditions_1: ionic strength: 0.05 M; pH: 6.2; pressure: 1 atm; temperature: 303 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY v3.113, Goddard - data analysis
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
BMRB | 16698 17663 |
PDB | |
EMBL | CAA45680 CAC84353 CAC84354 |
GB | AAA46125 AAA66558 |
PIR | WMBEHA |
REF | NP_040259 |
SP | P13199 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts