BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17699

Title: SOLUTION NMR STRUCTURE OF THE S4S5 LINKER OF HERG POTASSIUM CHANNEL   PubMed: 22359612

Deposition date: 2011-06-13 Original release date: 2012-03-02

Authors: Ng, C.; Kuchel, P.; Vandenberg, J.

Citation: Ng, Chai Ann; Perry, Matthew; Tan, Peter; Hill, Adam; Kuchel, Philip; Vandenberg, Jamie. "The s4-s5 linker acts as a signal integrator for HERG k channel activation and deactivation gating."  PLoS ONE 7, e31640-. (2012).

Assembly members:
S4S5_Linker, polymer, 20 residues, 2330.752 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
S4S5_Linker: LVRVARKLDRYSEYGAAVLF

Data sets:
Data typeCount
1H chemical shifts212

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SOLUTION NMR STRUCTURE OF THE S4S5 LINKER OF HERG POTASSIUM CHANNEL1

Entities:

Entity 1, SOLUTION NMR STRUCTURE OF THE S4S5 LINKER OF HERG POTASSIUM CHANNEL 20 residues - 2330.752 Da.

1   LEUVALARGVALALAARGLYSLEUASPARG
2   TYRSERGLUTYRGLYALAALAVALLEUPHE

Samples:

sample: DPC, {U-2H], 100 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.6; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsampleisotropicsample_conditions_1
2D TOCSYsampleisotropicsample_conditions_1

Software:

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - geometry optimization, structure solution

AMBER v11, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

XEASY, Bartels et al. - chemical shift assignment, data analysis, peak picking

TOPSPIN v3, Bruker Biospin - collection, processing

NMR spectrometers:

  • BRUKER Avance 800 MHz

Related Database Links:

PDB
DBJ BAA37096 BAC03764 BAG59132 BAG60605
EMBL CAA09232 CAB09536 CAB64868 CAB66135 CAC14797
GB AAA62473 AAB87571 AAB94741 AAB94742 AAC48722
REF NP_000229 NP_001003145 NP_001032801 NP_001036187 NP_001092571
SP O08703 O08962 O35219 O54852 O54853
TPG DAA18406 DAA18407 DAA30311 DAA32713