BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17970

Title: Solution structure and binding studies of the RanBP2-type zinc finger of RBM5   PubMed: 22162216

Deposition date: 2011-09-30 Original release date: 2012-01-05

Authors: Farina, Biancamaria; Pellecchia, Maurizio

Citation: Farina, Biancamaria; Fattorusso, Roberto; Pellecchia, Maurizio. "Targeting Zinc Finger Domains with Small Molecules: Solution Structure and Binding Studies of the RanBP2-Type Zinc Finger of RBM5"  ChemBioChem 12, 2837-2845 (2011).

Assembly members:
RBM5-ZF1, polymer, 32 residues, 3734.461 Da.
AQN, non-polymer, 288.275 Da.
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Homo sapiens   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
RBM5-ZF1: XKFEDWLCNKCCLNNFRKRL KCFRCGADKFDX

Data sets:
Data typeCount
1H chemical shifts214

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RBM5-ZF11
2AQN2
3ZINC ION3

Entities:

Entity 1, RBM5-ZF1 32 residues - 3734.461 Da.

1   ACELYSPHEGLUASPTRPLEUCYSASNLYS
2   CYSCYSLEUASNASNPHEARGLYSARGLEU
3   LYSCYSPHEARGCYSGLYALAASPLYSPHE
4   ASPNH2

Entity 2, AQN - C14 H8 O5 S - 288.275 Da.

1   AQN

Entity 3, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: RBM5-ZF1 100 uM; ZINC ION 150 uM; AQN 500 uM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0 M; pH: 5.7; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN vv2.1, Bruker Biospin - collection, data analysis

CARA vv1.5,v1.8, Keller and Wuthrich - chemical shift assignment, peak picking

CYANA vv2.1, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

SYBYL, Tripos - Energy Minimization, refinement

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

BMRB 17387 17969
PDB
DBJ BAE22042 BAE27501 BAE87995 BAG36742 BAG59728
EMBL CAC69136
GB AAA99715 AAB42216 AAD04159 AAF02422 AAH19766
REF NP_001039839 NP_001094018 NP_001248103 NP_005769 NP_683732
SP B2GV05 P52756 Q1RMU5 Q91YE7
TPG DAA16943