BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17974

Title: Structural and mechanistic insights into the interaction between PAT Pyk2 and Paxillin LD motif

Deposition date: 2011-10-04 Original release date: 2012-10-09

Authors: Vanarotti, Murugendra; Miller, Darcie; Guibao, Cristina; Zheng, Jie

Citation: Vanarotti, Murugendra; Miller, Darcie; Guibao, Cristina; Zheng, Jie. "Structural and mechanistic insights into the interaction between PAT Pyk2 and Paxillin LD motif"  Not known ., .-..

Assembly members:
entity, polymer, 135 residues, 14815.178 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: ANLDRTDDLVYLNVMELVRA VLELKNELSQLPPEGYVVVV KNVGLTLRKLIGSVDDLLPS LPSSSRTEIEGTQKLLNKDL AELINKMRLAQQNAVTSLSE EAKRQMLTASHTLAVDAKNL LDAVDQAKVLANLAH

Data sets:
Data typeCount
13C chemical shifts438
15N chemical shifts143
1H chemical shifts998

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PAT Pyk2 and Paxillin LD motif1

Entities:

Entity 1, PAT Pyk2 and Paxillin LD motif 135 residues - 14815.178 Da.

1   ALAASNLEUASPARGTHRASPASPLEUVAL
2   TYRLEUASNVALMETGLULEUVALARGALA
3   VALLEUGLULEULYSASNGLULEUSERGLN
4   LEUPROPROGLUGLYTYRVALVALVALVAL
5   LYSASNVALGLYLEUTHRLEUARGLYSLEU
6   ILEGLYSERVALASPASPLEULEUPROSER
7   LEUPROSERSERSERARGTHRGLUILEGLU
8   GLYTHRGLNLYSLEULEUASNLYSASPLEU
9   ALAGLULEUILEASNLYSMETARGLEUALA
10   GLNGLNASNALAVALTHRSERLEUSERGLU
11   GLUALALYSARGGLNMETLEUTHRALASER
12   HISTHRLEUALAVALASPALALYSASNLEU
13   LEUASPALAVALASPGLNALALYSVALLEU
14   ALAASNLEUALAHIS

Samples:

sample_1: MES, [U-100% 13C; U-100% 15N], 0.5 – 1 mM; H2O, natural source, 90%; D2O, natural source, 10%

sample_conditions_1: ionic strength: 7 mM; pH: 6.2; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAI45770
EMBL CAH92304
GB AAB35701 AAB47217 AAC05330 AAC50203 AAH36651
PRF 2119367A 2208337A
REF NP_001095722 NP_001127536 NP_004094 NP_775266 NP_775267
SP Q14289
TPG DAA26677

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts