BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17990

Title: STRUCTURES AND INTERACTIONS ANALYSES OF THE INTEGRIN ALPHA-M BETA-2 CYTOPLASMIC TAILS   PubMed: 22052909

Deposition date: 2011-10-11 Original release date: 2011-11-09

Authors: Chua, Geok Lin; Tang, Xiao Yan; Amalraj, Monalisa; Bhattacharjya, Surajit; Tan, Suet Mien

Citation: Chua, Geok-Lin; Tang, Xiao-Yan; Amalraj, Monalisa; Tan, Suet-Mien; Bhattacharjya, Surajit. "Structures and interaction analyses of integrin M2 cytoplasmic tails."  J. Biol. Chem. 286, 43842-43854 (2011).

Assembly members:
entity, polymer, 24 residues, 2703.118 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity: KLGFFKRQYKDMMSEGGPPG AEPQ

Data sets:
Data typeCount
13C chemical shifts22
1H chemical shifts132

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MYRISTOYLATED ALPHA M1

Entities:

Entity 1, MYRISTOYLATED ALPHA M 24 residues - 2703.118 Da.

INTEGRIN MAC-1 ALPHA CYTOPLASMIC TAIL

1   LYSLEUGLYPHEPHELYSARGGLNTYRLYS
2   ASPMETMETSERGLUGLYGLYPROPROGLY
3   ALAGLUPROGLN

Samples:

MYR-ALPHA-M: MAC-1 0.7 mM; deuterated Dodecylphosphocholine, [U-99% 2H], 200 mM; sodium phosphate 10 mM; H2O 90%; D2O, [U-99% 2H], 10%

MYR-ALPHA-M_D2O: MAC-1 0.7 mM; deuterated Dodecylphosphocholine, [U-99% 2H], 200 mM; sodium phosphate 10 mM; D2O, [U-99% 2H], 100%

sample_conditions_1: ionic strength: 10 mM; pH: 5.6; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYMYR-ALPHA-Misotropicsample_conditions_1
2D 1H-1H NOESYMYR-ALPHA-Misotropicsample_conditions_1
2D 1H-1H NOESYMYR-ALPHA-M_D2Oisotropicsample_conditions_1
2D 1H-13C HSQCMYR-ALPHA-M_D2Oisotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

TOPSPIN v1.3, Bruker Biospin - processing

SPARKY v3.11, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAE91421 BAG10590 BAG54538
GB AAA59491 AAA59544 AAA59903 AAB24821 AAH96346
REF NP_000623 NP_001139280 NP_001181863 XP_003280535 XP_004692013
SP P11215