BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18118

Title: NMR structure of the protein BC040485 from Homo sapiens

Deposition date: 2011-12-01 Original release date: 2012-01-25

Authors: Dutta, Samit; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt; JCSG, JCSG

Citation: Dutta, Samit; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt. "NMR structure of the human RNA binding protein BC040485"  Not known ., .-..

Assembly members:
entity, polymer, 107 residues, 12443.094 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: GSKLEEEVDDVFLIRAQGLP WSCTMEDVLNFFSDCRIRNG ENGIHFLLNRDGKRRGDALI EMESEQDVQKALEKHRMYMG QRYVEVYEINNEDVDALMKS LQVKSSP

Data sets:
Data typeCount
13C chemical shifts340
15N chemical shifts113
1H chemical shifts698

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BC0404851

Entities:

Entity 1, BC040485 107 residues - 12443.094 Da.

1   GLYSERLYSLEUGLUGLUGLUVALASPASP
2   VALPHELEUILEARGALAGLNGLYLEUPRO
3   TRPSERCYSTHRMETGLUASPVALLEUASN
4   PHEPHESERASPCYSARGILEARGASNGLY
5   GLUASNGLYILEHISPHELEULEUASNARG
6   ASPGLYLYSARGARGGLYASPALALEUILE
7   GLUMETGLUSERGLUGLNASPVALGLNLYS
8   ALALEUGLULYSHISARGMETTYRMETGLY
9   GLNARGTYRVALGLUVALTYRGLUILEASN
10   ASNGLUASPVALASPALALEUMETLYSSER
11   LEUGLNVALLYSSERSERPRO

Samples:

sample_1: entity, [U-98% 13C; U-98% 15N], 1.2 mM; sodium phosphate 20 mM; sodium azide 5 mM; sodium chloride 50 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.080 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
4D HACANHsample_1isotropicsample_conditions_1
5D HACACONHsample_1isotropicsample_conditions_1
5D CBCACONHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

CYANA, G ntert P. - structure solution

TOPSPIN, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment, data analysis

OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts