BMRB Entry 18137
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18137
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Title: NMR assignment of the monomeric DUF59 domain of human Fam96a PubMed: 22618863
Deposition date: 2011-12-12 Original release date: 2012-01-09
Authors: Mas, Caroline; Chen, Kai-En; Brereton, Ian; Martin, Jennifer; Hill, Justine
Citation: Mas, Caroline; Chen, Kai-En; Brereton, Ian; Martin, Jennifer; Hill, Justine. "Backbone resonance assignments of the monomeric DUF59 domain of human Fam96a." Biomol. NMR Assignments 7, 117-120 (2013).
Assembly members:
Fam96a, polymer, 130 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Fam96a: SNARIMEEKALEVYDLIRTI
RDPEKPNTLEELEVVSESCV
EVQEINEEEYLVIIRFTPTV
PHCSLATLIGLCLRVKLQRC
LPFKHKLEIYISEGTHSTEE
DINKQINDKERVAAAMENPN
LREIVEQCVL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 460 |
| 15N chemical shifts | 105 |
| 1H chemical shifts | 103 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Fam96a | 1 |
Entities:
Entity 1, Fam96a 130 residues - Formula weight is not available
| 1 | SER | ASN | ALA | ARG | ILE | MET | GLU | GLU | LYS | ALA | |
| 2 | LEU | GLU | VAL | TYR | ASP | LEU | ILE | ARG | THR | ILE | |
| 3 | ARG | ASP | PRO | GLU | LYS | PRO | ASN | THR | LEU | GLU | |
| 4 | GLU | LEU | GLU | VAL | VAL | SER | GLU | SER | CYS | VAL | |
| 5 | GLU | VAL | GLN | GLU | ILE | ASN | GLU | GLU | GLU | TYR | |
| 6 | LEU | VAL | ILE | ILE | ARG | PHE | THR | PRO | THR | VAL | |
| 7 | PRO | HIS | CYS | SER | LEU | ALA | THR | LEU | ILE | GLY | |
| 8 | LEU | CYS | LEU | ARG | VAL | LYS | LEU | GLN | ARG | CYS | |
| 9 | LEU | PRO | PHE | LYS | HIS | LYS | LEU | GLU | ILE | TYR | |
| 10 | ILE | SER | GLU | GLY | THR | HIS | SER | THR | GLU | GLU | |
| 11 | ASP | ILE | ASN | LYS | GLN | ILE | ASN | ASP | LYS | GLU | |
| 12 | ARG | VAL | ALA | ALA | ALA | MET | GLU | ASN | PRO | ASN | |
| 13 | LEU | ARG | GLU | ILE | VAL | GLU | GLN | CYS | VAL | LEU |
Samples:
sample_1: Fam96a, [U-100% 13C; U-100% 15N], 0.8 mM; Hepes buffer 25 mM; NaCl 150 mM; DTT 2 mM
sample_conditions_1: ionic strength: 150 mM; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D CC-TOCSY-CONH | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis
TOPSPIN, Bruker Biospin - collection
CCcpNMR, CCPN - chemical shift assignment
NMR spectrometers:
- Bruker Avance 750 MHz
- Bruker Avance 900 MHz
Related Database Links:
| UNP | Q9H5X1 |
| BMRB | 18548 |
| PDB | |
| DBJ | BAB15496 BAG35105 BAH14131 |
| GB | AAH08865 AAI02257 AAI51429 ABQ82139 AIC57308 |
| REF | NP_001014812 NP_001030282 NP_001092912 NP_001276037 NP_115607 |
| SP | Q3T0U7 Q9H5X1 |
| TPG | DAA25322 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts