BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18197

Title: rat Angiogenin   PubMed: 22477091

Deposition date: 2012-01-16 Original release date: 2012-05-11

Authors: Lee, Chung-Kyung; Cheong, Hae-Kap

Citation: Lee, Chung-Kyung; Yeo, Kwon Joo; Hwang, Eunha; Cheong, Hae-Kap. "(1)H, (13)C, (15)N backbone and side-chain resonance assignments of rat angiogenin."  Biomol. NMR Assignments 7, 89-92 (2013).

Assembly members:
angiogenin, polymer, 126 residues, Formula weight is not available

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
angiogenin: GSHMAQDDPRYTKFLTQHYD AKPKGRDARYCESMMRRRGL TSPCKEVNTFIHGNKGSIKA ICGANGSPYGENLRISQSPF QITTCKHTGGSPRPPCRYRA SAGFRHVVIACENGLPVHFD ESFISL

Data sets:
Data typeCount
13C chemical shifts482
15N chemical shifts123
1H chemical shifts767

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1angiogenin1

Entities:

Entity 1, angiogenin 126 residues - Formula weight is not available

1   GLYSERHISMETALAGLNASPASPPROARG
2   TYRTHRLYSPHELEUTHRGLNHISTYRASP
3   ALALYSPROLYSGLYARGASPALAARGTYR
4   CYSGLUSERMETMETARGARGARGGLYLEU
5   THRSERPROCYSLYSGLUVALASNTHRPHE
6   ILEHISGLYASNLYSGLYSERILELYSALA
7   ILECYSGLYALAASNGLYSERPROTYRGLY
8   GLUASNLEUARGILESERGLNSERPROPHE
9   GLNILETHRTHRCYSLYSHISTHRGLYGLY
10   SERPROARGPROPROCYSARGTYRARGALA
11   SERALAGLYPHEARGHISVALVALILEALA
12   CYSGLUASNGLYLEUPROVALHISPHEASP
13   GLUSERPHEILESERLEU

Samples:

sample_1: angiogenin, [U-100% 13C; U-100% 15N], 1.0 mM; MES 50 mM; sodium chloride 100 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
GB AAR28758 AAV87193 EDL88436 EDL88438
REF NP_001006993 XP_006251936
TPE CDG32031

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts