BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18254

Title: 1H and 15N resonance assignments for Pseudomonas aeruginosa apo-azurin   PubMed: 22645370

Deposition date: 2012-02-10 Original release date: 2012-09-14

Authors: Zaballa, Maria-Eugenia; Abriata, Luciano; Donaire, Antonio; Vila, Alejandro

Citation: Zaballa, Maria-Eugenia; Abriata, Luciano; Donaire, Antonio; Vila, Alejandro. "Flexibility of the metal-binding region in apo-cupredoxins."  Proc. Natl. Acad. Sci. U.S.A. 109, 9254-9259 (2012).

Assembly members:
apo-azurin, polymer, 128 residues, Formula weight is not available

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
apo-azurin: AECSVDIQGNDQMQFNTNAI TVDKSCKQFTVNLSHPGNLP KNVMGHNWVLSTAADMQGVV TDGMASGLDKDYLKPDDSRV IAHTKLIGSGEKDSVTFDVS KLKEGEQYMFFCTFPGHSAL MKGTLTLK

Data sets:
Data typeCount
15N chemical shifts107
1H chemical shifts107

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apo-azurin1

Entities:

Entity 1, apo-azurin 128 residues - Formula weight is not available

1   ALAGLUCYSSERVALASPILEGLNGLYASN
2   ASPGLNMETGLNPHEASNTHRASNALAILE
3   THRVALASPLYSSERCYSLYSGLNPHETHR
4   VALASNLEUSERHISPROGLYASNLEUPRO
5   LYSASNVALMETGLYHISASNTRPVALLEU
6   SERTHRALAALAASPMETGLNGLYVALVAL
7   THRASPGLYMETALASERGLYLEUASPLYS
8   ASPTYRLEULYSPROASPASPSERARGVAL
9   ILEALAHISTHRLYSLEUILEGLYSERGLY
10   GLULYSASPSERVALTHRPHEASPVALSER
11   LYSLEULYSGLUGLYGLUGLNTYRMETPHE
12   PHECYSTHRPHEPROGLYHISSERALALEU
13   METLYSGLYTHRLEUTHRLEULYS

Samples:

sample_1: apoAzurin, [U-100% 13C; U-100% 15N], 1 mM; DTT 2 mM; potassium phosphate 100 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CARA, Rochus Keller - chemical shift assignment, data analysis

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 1210 1211 1212 1213 1214 1215 1216 1217 1218 1219 4465
PDB
DBJ BAK87490 BAP24727 BAP53499 BAQ42761 BAR70381
EMBL CAA30279 CAW30062 CCQ86145 CDH73668 CDH79986
GB AAA25730 AAG08307 AAP03090 AAT49489 ABJ14307
PRF 671048A
REF NP_253609 WP_003095591 WP_003141697 WP_012077620 WP_031690945
SP B3EWN9 P00282

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts