BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18357

Title: The solution structure of the monomeric Acanthaporin

Deposition date: 2012-03-28 Original release date: 2012-05-01

Authors: Michalek, Matthias; Soennichsen, Frank; Wechselberger, Rainer; Dingley, Andrew; Wienk, Hans; Simanski, Maren; Herbst, Rosa; Lorenzen, Inken; Marciano-Cabral, Francine; Gelhaus, Christoph; Groetzinger, Joachim; Leippe, Matthias

Citation: Michalek, Matthias; Soennichsen, Frank; Wechselberger, Rainer; Dingley, Andrew; Wienk, Hans; Simanski, Maren; Herbst, Rosa; Lorenzen, Inken; Marciano-Cabral, Francine; Gelhaus, Christoph; Groetzinger, Joachim; Leippe, Matthias. "The solution structure of the monomeric Acanthaporin"  Not known ., .-..

Assembly members:
acanthaporin, polymer, 61 residues, 6001.235 Da.

Natural source:   Common Name: Acanthamoeba culbertsoni   Taxonomy ID: 43142   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Acanthamoeba culbertsoni

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
acanthaporin: AMGKCSVLKKVACAAAIAGA VAACGGIDLPCVLAALKAAE GCASCFCEDHCHGVCKDLHL C

Data sets:
Data typeCount
1H chemical shifts319

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Acanthaporin1

Entities:

Entity 1, Acanthaporin 61 residues - 6001.235 Da.

1   ALAMETGLYLYSCYSSERVALLEULYSLYS
2   VALALACYSALAALAALAILEALAGLYALA
3   VALALAALACYSGLYGLYILEASPLEUPRO
4   CYSVALLEUALAALALEULYSALAALAGLU
5   GLYCYSALASERCYSPHECYSGLUASPHIS
6   CYSHISGLYVALCYSLYSASPLEUHISLEU
7   CYS

Samples:

sample_1: acanthaporin, [U-100% 15N], 1.0 mM; D2O, [U-2H], 7%; sodium phosphate buffer 50 mM; sodium azide 0.001%; H2O 93%

sample_conditions_1: ionic strength: 0.05 M; pH: 5.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Bruker Avance 750 MHz
  • Bruker Avance 600 MHz

Related Database Links:

BMRB 16727 16819 18358
PDB
GB AEC33273