BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18385

Title: Characterization of the chromoshadow domain-mediated binding of heterochromatin protein 1 alpha (HP1 alpha) to histone H3: Backbone 1H, 15N chemical shift assignments for histone H3 (1-59)   PubMed: 22493481

Deposition date: 2012-04-10 Original release date: 2012-05-21

Authors: Richart, Alexandria; Clair, Brunner; Katherine, Stott; Natalia, Murzina; Jean, Thomas

Citation: Richart, Alexandria; Brunner, Clair; Stott, Katherine; Murzina, Natalia; Thomas, Jean. "Characterization of Chromoshadow Domain-mediated Binding of Heterochromatin Protein 1 (HP1) to Histone H3."  J. Biol. Chem. 287, 18730-18737 (2012).

Assembly members:
H3(1-59), polymer, 59 residues, Formula weight is not available

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
H3(1-59): ARTKQTARKSTGGKAPRKQL ATKAARKSAPATGGVKKPHR YRPGTVALREIRRYQKSTE

Data sets:
Data typeCount
15N chemical shifts55
1H chemical shifts55

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1H3(1-59)1

Entities:

Entity 1, H3(1-59) 59 residues - Formula weight is not available

1   ALAARGTHRLYSGLNTHRALAARGLYSSER
2   THRGLYGLYLYSALAPROARGLYSGLNLEU
3   ALATHRLYSALAALAARGLYSSERALAPRO
4   ALATHRGLYGLYVALLYSLYSPROHISARG
5   TYRARGPROGLYTHRVALALALEUARGGLU
6   ILEARGARGTYRGLNLYSSERTHRGLU

Samples:

sample_1: H3(1-59), [U-95% 15N], 0.5 mM; sodium phosphate 10 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.01 M; pH: 7.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

AZARA, Boucher - processing

Analysis, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAA20144 BAA93621 BAA93622 BAA93623 BAA93624
EMBL CAA24375 CAA24647 CAA24952 CAA25242 CAA25262
GB AAA19824 AAA29441 AAA29965 AAA30003 AAA30026
PIR A56580 A56618 A56654 I48113 I49397
PRF 0710252A 0806228A 1202289A 1920342A 2021267A
REF NP_001005101 NP_001005464 NP_001013074 NP_001014411 NP_001016636
SP P02299 P06352 P08898 P08903 P22843
TPG DAA16173 DAA16175 DAA16178 DAA16187 DAA16190

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts