BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18409

Title: 1H Chemical Shift Assignments for the second transmembrane domain from human copper transport 1   PubMed: 22615137

Deposition date: 2012-04-20 Original release date: 2012-05-22

Authors: Yang, Lei; Huang, Zhaowei; Li, Fei

Citation: Yang, Lei; Huang, Zhaowei; Li, Fei. "Structural insights into the transmembrane domains of human copper transporter 1."  J. Pept. Sci. 18, 449-455 (2012).

Assembly members:
entity, polymer, 29 residues, 3540.426 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: obtained from a vendor

Entity Sequences (FASTA):
entity: KHLLQTVLHIIQVVISYFLM LIFMTYNKK

Data sets:
Data typeCount
1H chemical shifts220

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1second transmembrane domain from human copper transport 11

Entities:

Entity 1, second transmembrane domain from human copper transport 1 29 residues - 3540.426 Da.

1   LYSHISLEULEUGLNTHRVALLEUHISILE
2   ILEGLNVALVALILESERTYRPHELEUMET
3   LEUILEPHEMETTHRTYRASNLYSLYS

Samples:

sample_1: hCtr1-TMD2 2 mM; 40% hexafluoroisopropanol (HFIP) aqueous solution 40%

sample_conditions_1: pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert P. - refinement

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

PDB
REF XP_012317279