BMRB Entry 18416
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18416
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Title: 1H,15N and 13C backbone and side chain chemical shifts of the ubiquitin homology domain of mouse BAG-1 PubMed: 22903788
Deposition date: 2012-04-24 Original release date: 2012-09-14
Authors: Huang, Hsiao Wen; Yu, Chin
Citation: Huang, Hsiao-Wen; Yu, Chin. "Backbone and side-chain resonance assignments (1H, 15N and 13C) of the ubiquitin homology domain of mouse BAG-1." Biomol. NMR Assignments 7, 235-239 (2013).
Assembly members:
ubiquitin_homology_domain_of_mouse_BAG-1, polymer, 97 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ubiquitin_homology_domain_of_mouse_BAG-1: MAKTEEMVQTEEMETPRLSV
IVTHSNERYDLLVTPQQGNS
EPVVQDLAQLVEEATGVPLP
FQKLIFKGKSLKEMETPLSA
LGMQNGCRVMLIGEKSN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 383 |
| 15N chemical shifts | 96 |
| 1H chemical shifts | 634 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ubiquitin homology of mouse BAG-1 | 1 |
Entities:
Entity 1, ubiquitin homology of mouse BAG-1 97 residues - Formula weight is not available
| 1 | MET | ALA | LYS | THR | GLU | GLU | MET | VAL | GLN | THR | ||||
| 2 | GLU | GLU | MET | GLU | THR | PRO | ARG | LEU | SER | VAL | ||||
| 3 | ILE | VAL | THR | HIS | SER | ASN | GLU | ARG | TYR | ASP | ||||
| 4 | LEU | LEU | VAL | THR | PRO | GLN | GLN | GLY | ASN | SER | ||||
| 5 | GLU | PRO | VAL | VAL | GLN | ASP | LEU | ALA | GLN | LEU | ||||
| 6 | VAL | GLU | GLU | ALA | THR | GLY | VAL | PRO | LEU | PRO | ||||
| 7 | PHE | GLN | LYS | LEU | ILE | PHE | LYS | GLY | LYS | SER | ||||
| 8 | LEU | LYS | GLU | MET | GLU | THR | PRO | LEU | SER | ALA | ||||
| 9 | LEU | GLY | MET | GLN | ASN | GLY | CYS | ARG | VAL | MET | ||||
| 10 | LEU | ILE | GLY | GLU | LYS | SER | ASN |
Samples:
sample_1: ubiquitin homology domain of mouse BAG-1, [U-100% 13C; U-100% 15N], 1.0 mM; sodium phosphate 20 mM; sodium chloride 100 mM; DTT 5 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMR, Varian - collection, processing
SPARKY, Goddard - chemical shift assignment, data analysis
NMR spectrometers:
- Varian VNMR 700 MHz
Related Database Links:
| PDB | |
| DBJ | BAB26106 |
| GB | AAC34259 AAH03722 AAH69918 AAH93509 EDL05422 |
| REF | NP_001165210 NP_033866 |
| SP | Q60739 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts